A Simple Solution for Oligonucleotide Design



Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:

Total 54 Oligos
Common Data ID: JX0068 Virus: Variola virus; Monkeypox virus; Cowpox virus; Vaccinia virus (strain WR); Ectromelia virus; camelpox virus; volepox virus; Raccoonpox virus; skunkpox virus
PMID: 9079767Publication date:03/97 Type: ATI-PCR
Temp: (94C 1min>40C 1min>72C 2.5min)*25>72C 10min
Primer Name: ATI-up-1 Type of Usage: Tm: 44 C
Primer Name: ATI-low-1 Type of Usage: Tm: 46 C
Common Data ID: JX0086 Virus: Cowpox virus; Ectromelia virus; Taterapox virus; Vaccinia virus (strain Lister); Buffalopox virus; Rabbitpox virus (strain Utrecht); Variola major virus; variola minor virus; Monkeypox virus; Camelpox virus
PMID: 11136755Publication date:01/01 Type: Universal PCR
Temp: (94C 1min>55C 1min>72C 1min)*25>72C 5min
Primer Name: VL2N Type of Usage: Tm: 50 C
Primer Name: VL33 Type of Usage: Tm: 62.9 C at salt 1000.0mM
Common Data ID: MP0440 Virus: Vaccinia virus, VAC; Rabbitpox virus, RPV; Buffalopox virus, BFL; Ectromelia virus, ECT; Camelpox virus, CML; Taterapox virus, GBL; Cowpox virus, CPV; Variola virus, VAR; Monkeypox virus, MPV
PMID: 7559950Publication date:08/95 Type: Universal PCR
Temp: (94C 1min>55C 2min>72C 3min)*25
Primer Name: EACP1 Type of Usage: Tm: 67.2 C at salt 1000.0mM
Primer Name: EACP2 Type of Usage: Tm: 46 C
Common Data ID: MP0441 Virus: Variola virus, VAR
PMID: 7559950Publication date:08/95 Type: PCR
Temp: (94C 1min>55C 2min>72C 3min)*25
Primer Name: VAR1 Type of Usage: PCRTm: 46 C
Primer Name: VAR2 Type of Usage: PCRTm: 69.7 C at salt 1000.0mM
Common Data ID: SA0001 Virus: Variola virus
PMID: 15652214Publication date:2004 Type: Multiplex PCR
Temp: 93C 120sec>(93C 30sec>50C 45sec>72C 120sec)*30>72C 600sec
Primer Name: VARV Type of Usage: Forward PrimerTm: 72.6C at salt 1000.0 mM
Primer Name: VARVR Type of Usage: Reverse PrimerTm: 73.6C at salt 1000.0 mM
Common Data ID: SG0046 Virus: Variola virus
PMID: 14962998Publication date:2004 Type: Real-time PCR
Temp: 94C 360sec>(94C 5sec>51C 10sec>72C 15sec)*40
Primer Name: OPS3 Type of Usage: Forward PrimerTm: 65C at salt 1000.0 mM
Primer Name: OPAs4 Type of Usage: Reverse PrimerTm: 64.6C at salt 1000.0 mM
Primer Name: OPP1 Type of Usage: PCR ProbeTm: max Tm 79.5C min Tm 78.2C at salt 1000.0mM
Primer Name: OPP2-640 Type of Usage: PCR ProbeTm: 72.5C at salt 1000.0 mM
Common Data ID: SG0079 Virus: Variola virus
PMID: 15147587Publication date:05/04 Type: PCR
Temp: 95C 300sec>(95C 30sec>55C 30sec>72C 60sec)*35>72C 420sec
Primer Name: SG007901 Type of Usage: Forward PrimerTm: max Tm 72C min Tm 70C at salt 1000.0mM
Primer Name: SG007902 Type of Usage: Reverse PrimerTm: 66.1C at salt 1000.0 mM
Primer Name: SG007903 Type of Usage: Hybridization ProbeTm: 73.9C at salt 1000.0 mM
Primer Name: SG007907 Type of Usage: Forward PrimerTm: 67.3C at salt 1000.0 mM
Primer Name: SG007908 Type of Usage: Reverse PrimerTm: 70.5C at salt 1000.0 mM
Primer Name: SG007909 Type of Usage: Hybridization ProbeTm: 72.6C at salt 1000.0 mM
Common Data ID: SG0105 Virus: Variola virus
PMID: 15004077Publication date:03/04 Type: Real-time PCR
Temp: 95C 600sec>(95C 10sec>55C 10sec>72C 10sec)*45
Primer Name: rpo OPV F1 Type of Usage: Forward PrimerTm: 73.6C at salt 1000.0 mM
Primer Name: rpo OPV R1 Type of Usage: Reverse PrimerTm: 70.2C at salt 1000.0 mM
Primer Name: rpo Var302 Sen Type of Usage: Hybridization ProbeTm: 73.3C at salt 1000.0 mM
Primer Name: rpo Var302 Anc Type of Usage: Hybridization ProbeTm: 75.6C at salt 1000.0 mM
Primer Name: VETF OPV F1 Type of Usage: Forward PrimerTm: 74.5C at salt 1000.0 mM
Primer Name: VETF OPV R1 Type of Usage: Reverse PrimerTm: 72.3C at salt 1000.0 mM
Primer Name: VETF Var181 Sen Type of Usage: Hybridization ProbeTm: 73.6C at salt 1000.0 mM
Primer Name: VETF Var181 Anc Type of Usage: Hybridization ProbeTm: 77.9C at salt 1000.0 mM
Primer Name: A13L Var F Type of Usage: Forward PrimerTm: 71.5C at salt 1000.0 mM
Primer Name: A13L OPV F Type of Usage: Forward PrimerTm: 69.3C at salt 1000.0 mM
Primer Name: A13L FL Type of Usage: Forward PrimerTm: 76C at salt 1000.0 mM
Primer Name: A13L R iLC Type of Usage: Hybridization ProbeTm: 72.9C at salt 1000.0 mM
Common Data ID: SG0111 Virus: Variola major virus
PMID: 12904397Publication date:08/03 Type: Real-time PCR
Temp: 95C 120sec>(95C 10sec>60C 45sec)*45
Primer Name: OPXJ7R3U Type of Usage: Forward PrimerTm: 70.5C at salt 1000.0 mM
Primer Name: OPXJ7R3L Type of Usage: Reverse PrimerTm: 67.9C at salt 1000.0 mM
Primer Name: VARJ7R3P Type of Usage: PCR ProbeTm: 75.8C at salt 1000.0 mM
Common Data ID: SG0112 Virus: Variola major virus
PMID: 12904397Publication date:08/03 Type: Real-time PCR
Temp: 95C 120sec>(95C 1sec>60C 20sec)*45
Primer Name: OPXJ7R3U Type of Usage: Forward PrimerTm: 70.5C at salt 1000.0 mM
Primer Name: OPXJ7R3L Type of Usage: Reverse PrimerTm: 67.9C at salt 1000.0 mM
Primer Name: VARJ7R3P Type of Usage: PCR ProbeTm: 75.8C at salt 1000.0 mM
Common Data ID: SG0138 Virus: Variola virus
PMID: 15131152Publication date:05/04 Type: Real-time PCR
Temp: 95C 10sec>(95C 5sec>55C 15sec>72C 15sec)*45
Primer Name: Pox fw 14kd 1 Type of Usage: Forward PrimerTm: 74.7C at salt 1000.0 mM
Primer Name: Pox fw 14kd 2 Type of Usage: Forward PrimerTm: 74.7C at salt 1000.0 mM
Primer Name: Pox RT 14kb 1 Type of Usage: Reverse PrimerTm: 71.4C at salt 1000.0 mM
Primer Name: Pox RT 14kd 2 Type of Usage: Forward PrimerTm: 69.9C at salt 1000.0 mM
Primer Name: Anchor probe Pox Anc Type of Usage: PCR ProbeTm: 83.9C at salt 1000.0 mM
Primer Name: Sensor probe MGB pox 14kd Type of Usage: PCR ProbeTm: 66.6C at salt 1000.0 mM
Common Data ID: YS0012 Virus: Variola virus
PMID: 14624234Publication date:11/17/2003 Type: Hybridization
Temp:
Primer Name: 10000031 Type of Usage: Tm: 90.5C at salt 1000.0 mM
Primer Name: 10000032 Type of Usage: Tm: 90.5C at salt 1000.0 mM
Primer Name: 10000033 Type of Usage: Tm: 87.57C at salt 1000.0 mM
Primer Name: 10000034 Type of Usage: Tm: 87.57C at salt 1000.0 mM
Primer Name: 10000035 Type of Usage: Tm: 82.89C at salt 1000.0 mM
Primer Name: 10000036 Type of Usage: Tm: 82.89C at salt 1000.0 mM
Primer Name: 10000037 Type of Usage: Tm: 85.81C at salt 1000.0 mM
Primer Name: 10000038 Type of Usage: Tm: 85.81C at salt 1000.0 mM
Primer Name: 10000039 Type of Usage: Tm: 83.47C at salt 1000.0 mM
Primer Name: 10000040 Type of Usage: Tm: 83.47C at salt 1000.0 mM
Total 54 Oligos
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