VirOligo Search

A Simple Solution for Oligonucleotide Design

Click here for a list of viruses in the database Two search options: 1. Search by virus name and/or primer data 2. Search by PMID, VirOligoID, or taxonomy ID; Virus name: Oligo Sequence: Tm: °C± °C Oligo length: bp± bp PMID: Common Data ID: Taxonomy ID: GI Number:
	Total 230 Oligos

	Common Data ID: MP1093	Virus: SARS coronavirus, SARS
	PMID: 12765993	Publication date:06/03	Type: RT-PCR
	Temp: 95C 10min>(95C 10sec>57C 5sec>72C 9sec)*50

	Primer Name: coro3	Type of Usage: PCR	Tm: 52 C
	Primer Name: coro4	Type of Usage: PCR	Tm: 48 C

	Common Data ID: SA0002	Virus: SARS coronavirus
	PMID: 15070991	Publication date:12/03	Type: Nested RT-PCR
	Temp:

	Primer Name: BNIoutAs	Type of Usage: Reverse Primer	Tm: 74.7C at salt 1000.0 mM
	Primer Name: BNIoutS2	Type of Usage: PCR	Tm: 70.2 C at salt 1000.0mM
	Primer Name: BNlinS	Type of Usage: PCR	Tm: 72 C at salt 1000.0mM
	Primer Name: BNIinAs	Type of Usage: PCR	Tm: 72.9 C at salt 1000.0mM

	Common Data ID: SA0003	Virus: SARS coronavirus
	PMID: 15070991	Publication date:12/03	Type: RT-PCR
	Temp:

	Primer Name: BNIoutAs	Type of Usage: Reverse Primer	Tm: 74.7C at salt 1000.0 mM
	Primer Name: Cor-p-F2 (+)	Type of Usage: PCR	Tm: 68.5 C at salt 1000.0mM
	Primer Name: Cor-p-R1 (–)	Type of Usage:	Tm: 70.5 C at salt 1000.0mM
	Primer Name: BNIoutS2	Type of Usage: PCR	Tm: 70.2 C at salt 1000.0mM

	Common Data ID: SA0004	Virus: SARS coronavirus
	PMID: 15070991	Publication date:12/03	Type: Real-time RT-PCR
	Temp:

	Primer Name: BNIoutAs	Type of Usage: Reverse Primer	Tm: 74.7C at salt 1000.0 mM
	Primer Name: BNIoutS2	Type of Usage: PCR	Tm: 70.2 C at salt 1000.0mM

	Common Data ID: SA0005	Virus: SARS coronavirus
	PMID: 15070991	Publication date:12/03	Type: RT-PCR
	Temp:

	Primer Name: SARSF	Type of Usage: Forward Primer	Tm: 70C at salt 1000.0 mM
	Primer Name: APNR	Type of Usage: Reverse Primer	Tm: 71.5C at salt 1000.0 mM

	Common Data ID: SA0006	Virus: SARS coronavirus
	PMID: 15070991	Publication date:12/03	Type: Real-time PCR
	Temp:

	Primer Name: SarsF APNF	Type of Usage: Forward Primer	Tm: 70C at salt 1000.0 mM
	Primer Name: Sars APNR 6	Type of Usage: Reverse Primer	Tm: 71.5C at salt 1000.0 mM
	Primer Name: Probe	Type of Usage: Hybridization Probe	Tm: 75C at salt 1000.0 mM

	Common Data ID: SC0003	Virus: SARS coronavirus
	PMID: 15184466	Publication date:06/04	Type: RT-PCR
	Temp: 61C 1200sec>95C 300sec>(95C 15sec>60C 30sec)*45

	Primer Name: NCCORFP	Type of Usage: Forward Primer	Tm: 69.3C at salt 1000.0 mM
	Primer Name: NCCORRP	Type of Usage: Reverse Primer	Tm: 71.5C at salt 1000.0 mM
	Primer Name: NCCORP	Type of Usage: Hybridization Probe	Tm: 76.8C at salt 1000.0 mM

	Common Data ID: SG0041	Virus: SARS coronavirus Tor2
	PMID: 14652014	Publication date:2003	Type: PCR
	Temp: 95C 180sec>(95C 10sec>60C 10sec[-1C/Cyc]>72C 20sec)10>(95C 10sec>56C 10sec>72C 20sec)40

	Primer Name: SARS1s	Type of Usage: Forward Primer	Tm: 74.4C at salt 1000.0 mM
	Primer Name: SARS1as	Type of Usage: Reverse Primer	Tm: 74.4C at salt 1000.0 mM
Primer Name: COR-1	Type of Usage: Forward Primer	Tm: 77.3C at salt 1000.0 mM
Primer Name: COR-2	Type of Usage: Reverse Primer	Tm: 72.6C at salt 1000.0 mM
Primer Name: BNIoutS2	Type of Usage: Forward Primer	Tm: 70.2C at salt 1000.0 mM
Primer Name: BNIoutAs	Type of Usage: Reverse Primer	Tm: 74.7C at salt 1000.0 mM

Common Data ID: SG0042	Virus: SARS coronavirus Tor2
PMID: 14652014	Publication date:2003	Type: Real-time PCR
Temp: 50C 120sec>95C 600sec>(95C 15sec>58C 60sec)*40

Primer Name: Primer C	Type of Usage: Reverse Primer	Tm: 74.7C at salt 1000.0 mM
Primer Name: Primer D	Type of Usage: Forward Primer	Tm: 73.7C at salt 1000.0 mM
Primer Name: Probe E	Type of Usage: PCR Probe	Tm: 78.6C at salt 1000.0 mM

Common Data ID: SG0044	Virus: SARS coronavirus HKU-39849
PMID: 14709637	Publication date:2004	Type: Real-time RT-PCR
Temp: 50C 120sec>95C 600sec>(95C 15sec>60C 60sec)*45

Primer Name: SG004401	Type of Usage: Forward Primer	Tm: 73.9C at salt 1000.0 mM
Primer Name: SG004402	Type of Usage: Reverse Primer	Tm: 75.5C at salt 1000.0 mM
Primer Name: SG004403	Type of Usage: PCR Probe	Tm: 50C
Primer Name: SG004404	Type of Usage: Forward Primer	Tm: 74.4C at salt 1000.0 mM
Primer Name: SG004405	Type of Usage: Reverse Primer	Tm: 75.5C at salt 1000.0 mM
Primer Name: SG004406	Type of Usage: PCR Probe	Tm: 50C

Common Data ID: SG0045	Virus: SARS coronavirus CUHK-W1
PMID: 14633867	Publication date:2003	Type: Real-time RT-PCR
Temp: 95C 300sec>(94C 20sec>56C 60sec)*40

Primer Name: SG004501	Type of Usage: Forward Primer	Tm: 73.2C at salt 1000.0 mM
Primer Name: SG004502	Type of Usage: Reverse Primer	Tm: 71C at salt 1000.0 mM
Primer Name: SG004503	Type of Usage: PCR Probe	Tm: 78.7C at salt 1000.0 mM
Primer Name: SG004504	Type of Usage: Forward Primer	Tm: 52C
Primer Name: SG004505	Type of Usage: Reverse Primer	Tm: 72.5C at salt 1000.0 mM
Primer Name: SG004506	Type of Usage: PCR Probe	Tm: 75.3C at salt 1000.0 mM

Common Data ID: SG0047	Virus: SARS coronavirus GD69
PMID: 14699465	Publication date:12/03	Type: Nested RT-PCR
Temp: 61C 1200sec>95C 30sec>(95C 1sec>55C 10sec>72C 8sec)*25

Primer Name: BNIoutS	Type of Usage: Forward Primer	Tm: 70.2C at salt 1000.0 mM
Primer Name: BNoutAs	Type of Usage: Reverse Primer	Tm: 74.7C at salt 1000.0 mM

Common Data ID: SG0048	Virus: SARS coronavirus GD69
PMID: 14699465	Publication date:12/03	Type: Nested RT-PCR
Temp: 95C 600sec>(95C 10sec>56C 5sec>72C 5sec)*35

Primer Name: BNIinS	Type of Usage: Forward Primer	Tm: 72C at salt 1000.0 mM
Primer Name: BNIAs	Type of Usage: Reverse Primer	Tm: 72.9C at salt 1000.0 mM

Common Data ID: SG0057	Virus: SARS coronavirus BJ03
PMID: 15080310	Publication date:2004	Type: In situ hybridization
Temp:

Primer Name: REP1	Type of Usage: Hybridization Probe	Tm: 83.3C at salt 1000.0 mM
Primer Name: REP2	Type of Usage: Hybridization Probe	Tm: 87.1C at salt 1000.0 mM
Primer Name: REP3	Type of Usage: Hybridization Probe	Tm: 88.6C at salt 1000.0 mM
Primer Name: REP4	Type of Usage: Hybridization Probe	Tm: 85C at salt 1000.0 mM
Primer Name: REP5	Type of Usage: Hybridization Probe	Tm: 85C at salt 1000.0 mM
Primer Name: M1	Type of Usage: Hybridization Probe	Tm: 83.1C at salt 1000.0 mM
Primer Name: M2	Type of Usage: Hybridization Probe	Tm: 85C at salt 1000.0 mM
Primer Name: M3	Type of Usage: Hybridization Probe	Tm: 84.1C at salt 1000.0 mM
Primer Name: N1	Type of Usage: Hybridization Probe	Tm: 84.1C at salt 1000.0 mM
Primer Name: N2	Type of Usage: Hybridization Probe	Tm: 88.4C at salt 1000.0 mM
Primer Name: N3	Type of Usage: Hybridization Probe	Tm: 82.9C at salt 1000.0 mM

Common Data ID: SG0062	Virus: SARS coronavirus FRA
PMID: 15030701	Publication date:02/04	Type: Real-time PCR
Temp: 50C 120sec>95C 600sec>(95C 15sec>61C 60sec)*45

Primer Name: Taq-772F	Type of Usage: Forward Primer	Tm: 72C at salt 1000.0 mM
Primer Name: Taq-1000R	Type of Usage: Reverse Primer	Tm: 72C at salt 1000.0 mM
Primer Name: Taq-955T	Type of Usage: PCR Probe	Tm: 78.8C at salt 1000.0 mM

Common Data ID: SG0063	Virus: SARS coronavirus
PMID: 15030702	Publication date:02/02	Type: RT-PCR
Temp: 50C 1800sec>95C 900sec>(95C 30sec>50C 40sec>72C 60sec)*40

Primer Name: Cor-p-F2 (+)	Type of Usage: PCR	Tm: 68.5 C at salt 1000.0mM
Primer Name: Cor-p-F3 (+)	Type of Usage: PCR	Tm: 76.4 C at salt 1000.0mM
Primer Name: Cor-p-R1 (–)	Type of Usage:	Tm: 70.5 C at salt 1000.0mM

Common Data ID: SG0064	Virus: SARS coronavirus
PMID: 15030702	Publication date:02/02	Type: Real-time RT-PCR
Temp: 50C 600sec>95C 600sec>(95C 2sec>55C 12sec>72C 10sec)*45

Primer Name: Cor-p-F2 (+)	Type of Usage: PCR	Tm: 68.5 C at salt 1000.0mM
Primer Name: Cor-p-F3 (+)	Type of Usage: PCR	Tm: 76.4 C at salt 1000.0mM
Primer Name: Cor-p-R1 (–)	Type of Usage:	Tm: 70.5 C at salt 1000.0mM

Common Data ID: SG0065	Virus: SARS coronavirus FRA
PMID: 14718079	Publication date:11/03	Type: Q-RT-PCR
Temp: 94C 180sec>(94C 30sec>60C 30sec>72C 60sec)*45>72C 7min

Primer Name: COR-1	Type of Usage: Forward Primer	Tm: 77.3C at salt 1000.0 mM
Primer Name: COR-2	Type of Usage: Reverse Primer	Tm: 72.6C at salt 1000.0 mM

Common Data ID: SG0070	Virus: SARS coronavirus FRA
PMID: 15030703	Publication date:02/04	Type: Real-time RT-PCR
Temp: 48C 1800sec>95C 600sec>(95C 15sec>60C 60sec)*45

Primer Name: SG007001	Type of Usage: Forward Primer	Tm: 74.7C at salt 1000.0 mM
Primer Name: SG007002	Type of Usage: Reverse Primer	Tm: 76.2C at salt 1000.0 mM
Primer Name: SG007003	Type of Usage: PCR Probe	Tm: 79C at salt 1000.0 mM
Primer Name: SG007004	Type of Usage: Forward Primer	Tm: 74.7C at salt 1000.0 mM
Primer Name: SG007005	Type of Usage: Reverse Primer	Tm: 56C
Primer Name: SG007006	Type of Usage: PCR Probe	Tm: 78.3C at salt 1000.0 mM
Primer Name: SG007007	Type of Usage: Forward Primer	Tm: 67.2C at salt 1000.0 mM
Primer Name: SG007008	Type of Usage: PCR Probe	Tm: 70.5C at salt 1000.0 mM
Primer Name: SG007009	Type of Usage: PCR Probe	Tm: 78.9C at salt 1000.0 mM
Primer Name: SG007010	Type of Usage: Forward Primer	Tm: 76.4C at salt 1000.0 mM
Primer Name: SG007011	Type of Usage: Reverse Primer	Tm: 60C
Primer Name: SG007012	Type of Usage: PCR Probe	Tm: 78.9C at salt 1000.0 mM
Primer Name: SG007013	Type of Usage: Forward Primer	Tm: 75.3C at salt 1000.0 mM
Primer Name: SG007014	Type of Usage: Forward Primer	Tm: 72C at salt 1000.0 mM
Primer Name: SG007015	Type of Usage: Forward Primer	Tm: 78.5C at salt 1000.0 mM
Primer Name: SG007016	Type of Usage: Forward Primer	Tm: 73.2C at salt 1000.0 mM
Primer Name: SG007017	Type of Usage: Reverse Primer	Tm: 71.5C at salt 1000.0 mM
Primer Name: SG007018	Type of Usage: PCR Probe	Tm: 79C at salt 1000.0 mM

Common Data ID: SG0072	Virus: SARS coronavirus TW1
PMID: 14519257	Publication date:09/03	Type: RT-PCR
Temp:

Primer Name: BNIinS	Type of Usage: Forward Primer	Tm: 72C at salt 1000.0 mM
Primer Name: BNIAS	Type of Usage: Reverse Primer	Tm: 72.9C at salt 1000.0 mM
Primer Name: Cor-p-F2	Type of Usage: Forward Primer	Tm: 68.5C at salt 1000.0 mM
Primer Name: Cor-p-R1	Type of Usage: Reverse Primer	Tm: 70.5C at salt 1000.0 mM
Primer Name: IN-6	Type of Usage: Forward Primer	Tm: 75C at salt 1000.0 mM
Primer Name: IN-7	Type of Usage: Reverse Primer	Tm: 70.2C at salt 1000.0 mM

Common Data ID: SG0073	Virus: SARS coronavirus GD69
PMID: 15078592	Publication date:01/04	Type: RT-PCR
Temp:

Primer Name: BNIoutS	Type of Usage: Forward Primer	Tm: 70.2C at salt 1000.0 mM
Primer Name: BNoutAs	Type of Usage: Reverse Primer	Tm: 74.7C at salt 1000.0 mM
Primer Name: BNIinS	Type of Usage: Forward Primer	Tm: 72C at salt 1000.0 mM
Primer Name: BNIAs	Type of Usage: Reverse Primer	Tm: 72.9C at salt 1000.0 mM
Primer Name: SARS1s	Type of Usage: Forward Primer	Tm: 74.4C at salt 1000.0 mM
Primer Name: SAR1as	Type of Usage: Reverse Primer	Tm: 74.4C at salt 1000.0 mM

Common Data ID: SG0074	Virus: SARS coronavirus TW1
PMID: 15109406	Publication date:03/04	Type: RT-PCR
Temp: 95C 180sec>(95C 10sec>60C 10sec [-1C/cycle]>72C 30sec)10>(95C 10sec>56C 10sec>72C 30sec)40

Primer Name: BNIoutS	Type of Usage: Forward Primer	Tm: 70.2C at salt 1000.0 mM
Primer Name: BNoutAs	Type of Usage: Reverse Primer	Tm: 74.7C at salt 1000.0 mM
Primer Name: BNIinS	Type of Usage: Forward Primer	Tm: 72C at salt 1000.0 mM
Primer Name: BNIAs	Type of Usage: Reverse Primer	Tm: 72.9C at salt 1000.0 mM

Common Data ID: SG0075	Virus: SARS coronavirus GD69
PMID: 15109406	Publication date:03/04	Type: Real-time RT-PCR
Temp:

Primer Name: BNITMSARS1	Type of Usage: Forward Primer	Tm: 72C at salt 1000.0 mM
Primer Name: BNITMSARAS2	Type of Usage: Reverse Primer	Tm: 76.6C at salt 1000.0 mM
Primer Name: BNI-TMSARP	Type of Usage: PCR Probe	Tm: 77C at salt 1000.0 mM

Common Data ID: SG0076	Virus: SARS coronavirus GD69
PMID: 15030711	Publication date:02/04	Type: RT-PCR
Temp:

Primer Name: BNIoutS	Type of Usage: Forward Primer	Tm: 70.2C at salt 1000.0 mM
Primer Name: BNoutAs	Type of Usage: Reverse Primer	Tm: 74.7C at salt 1000.0 mM
Primer Name: SARS1s	Type of Usage: Forward Primer	Tm: 74.4C at salt 1000.0 mM
Primer Name: SAR1as	Type of Usage: Reverse Primer	Tm: 74.4C at salt 1000.0 mM
Primer Name: COR-1	Type of Usage: Forward Primer	Tm: 77.3C at salt 1000.0 mM
Primer Name: COR-2	Type of Usage: Reverse Primer	Tm: 72.6C at salt 1000.0 mM

Common Data ID: SG0078	Virus: SARS coronavirus GD69
PMID: 14519240	Publication date:09/03	Type: RT-PCR
Temp: 50C 1800sec>94C 180sec>(94C 30sec>52C 30sec>72C 45sec)*40>72C 420sec

Primer Name: COR-1	Type of Usage: Forward Primer	Tm: 77.3C at salt 1000.0 mM
Primer Name: COR-2	Type of Usage: Reverse Primer	Tm: 72.6C at salt 1000.0 mM

Common Data ID: SG0086	Virus: SARS coronavirus FRA
PMID: 15135737	Publication date:2004	Type: RT-PCR
Temp: 50C 120sec>60C 1800sec>95C 300sec>(94C 15sec>58C 60sec)*50

Primer Name: SG008601	Type of Usage: Forward Primer	Tm: 73.9C at salt 1000.0 mM
Primer Name: SG008602	Type of Usage: Reverse Primer	Tm: 75.5C at salt 1000.0 mM
Primer Name: SG008603	Type of Usage: PCR Probe	Tm: 50C

Common Data ID: SG0090	Virus: SARS coronavirus
PMID: 14522060	Publication date:2003	Type: Real-time RT-PCR
Temp: 50C 120sec>95C 600sec>(95C 15sec>60C 60sec)*45

Primer Name: SG009001	Type of Usage: Forward Primer	Tm: 73.9C at salt 1000.0 mM
Primer Name: SG009002	Type of Usage: Reverse Primer	Tm: 75.5C at salt 1000.0 mM
Primer Name: SG009003	Type of Usage: PCR Probe	Tm: 50C

Common Data ID: SG0091	Virus: SARS coronavirus
PMID: 14522061	Publication date:2003	Type: Nested PCR
Temp: 95C 180sec>(95C 10sec>60C [+1C/cyc] 10sec>72C 20sec)10>(95C 10sec> 56C 10sec>72C 20sec)40

Primer Name: BNIoutS2	Type of Usage: Forward Primer	Tm: 70.2C at salt 1000.0 mM
Primer Name: BNIoutAs	Type of Usage: Reverse Primer	Tm: 74.7C at salt 1000.0 mM
Primer Name: BNIinS	Type of Usage: Forward Primer	Tm: 72C at salt 1000.0 mM
Primer Name: BNIAs	Type of Usage: Reverse Primer	Tm: 72.9C at salt 1000.0 mM

Common Data ID: SG0099	Virus: SARS coronavirus
PMID: 14715775	Publication date:01/04	Type: Nested RT-PCR
Temp: 94C 10sec>(94C 10sec>50C 30sec>72C 60sec)*35>72C 420sec

Primer Name: SG009901	Type of Usage: Forward Primer	Tm: 68.5C at salt 1000.0 mM
Primer Name: SG009902	Type of Usage: Reverse Primer	Tm: 68.5C at salt 1000.0 mM
Primer Name: SG009903	Type of Usage: Reverse Primer	Tm: 71.9C at salt 1000.0 mM
Primer Name: SG009904	Type of Usage: Forward Primer	Tm: 71.5C at salt 1000.0 mM

Common Data ID: SG0100	Virus: SARS coronavirus
PMID: 15004042	Publication date:03/04	Type: Real-time PCR
Temp: 50C 120sec>95C 600sec>(95C 15sec>60C 60sec)*40

Primer Name: SG010001	Type of Usage: Forward Primer	Tm: 74.7C at salt 1000.0 mM
Primer Name: SG010002	Type of Usage: Reverse Primer	Tm: 56C
Primer Name: SG010003	Type of Usage: PCR Probe	Tm: 78.9C at salt 1000.0 mM

Common Data ID: SG0101	Virus: SARS coronavirus
PMID: 15144898	Publication date:06/04	Type: Real-time RT-PCR
Temp:

Primer Name: ORF1-F	Type of Usage: Forward Primer	Tm: 76.1C at salt 1000.0 mM
Primer Name: ORF1-R	Type of Usage: Reverse Primer	Tm: 69.3C at salt 1000.0 mM
Primer Name: ORF1-probe	Type of Usage: PCR Probe	Tm: 77.1C at salt 1000.0 mM

Common Data ID: SG0118	Virus: SARS coronavirus
PMID: 15324554	Publication date:07/04	Type: Real-time RT-PCR
Temp: 50C 1800sec>95C 900sec>(95C 15sec>60C 30sec)*45

Primer Name: pp1a F	Type of Usage: Forward Primer	Tm: 76.8C at salt 1000.0 mM
Primer Name: pp1a R	Type of Usage: Reverse Primer	Tm: 75.5C at salt 1000.0 mM
Primer Name: pp1a TM	Type of Usage: PCR Probe	Tm: 80.6C at salt 1000.0 mM
Primer Name: NS F	Type of Usage: Forward Primer	Tm: 70.6C at salt 1000.0 mM
Primer Name: NS R	Type of Usage: Reverse Primer	Tm: 74.7C at salt 1000.0 mM
Primer Name: NS TM	Type of Usage: PCR Probe	Tm: 80.7C at salt 1000.0 mM
Primer Name: SS GP F	Type of Usage: Forward Primer	Tm: 75.5C at salt 1000.0 mM
Primer Name: SS GP R	Type of Usage: Reverse Primer	Tm: 70.5C at salt 1000.0 mM
Primer Name: SS GP TM	Type of Usage: Reverse Primer	Tm: 80.5C at salt 1000.0 mM

Common Data ID: SG0119	Virus: SARS coronavirus
PMID: 15324554	Publication date:07/04	Type: Real-time RT-PCR
Temp: 55C 1800sec>95C 30sec>(95C 1sec>55C 10sec>72C 10sec)*45

Primer Name: pp1a F	Type of Usage: Forward Primer	Tm: 76.8C at salt 1000.0 mM
Primer Name: pp1a R	Type of Usage: Reverse Primer	Tm: 75.5C at salt 1000.0 mM
Primer Name: pp1a TM	Type of Usage: PCR Probe	Tm: 80.6C at salt 1000.0 mM
Primer Name: NS F	Type of Usage: Forward Primer	Tm: 70.6C at salt 1000.0 mM
Primer Name: NS R	Type of Usage: Reverse Primer	Tm: 74.7C at salt 1000.0 mM
Primer Name: NS TM	Type of Usage: PCR Probe	Tm: 80.7C at salt 1000.0 mM
Primer Name: SS GP F	Type of Usage: Forward Primer	Tm: 75.5C at salt 1000.0 mM
Primer Name: SS GP R	Type of Usage: Reverse Primer	Tm: 70.5C at salt 1000.0 mM
Primer Name: SS GP TM	Type of Usage: Reverse Primer	Tm: 80.5C at salt 1000.0 mM

Common Data ID: SG0122	Virus: SARS coronavirus
PMID: 15030680	Publication date:02/04	Type: RT-PCR
Temp: 50C 1800sec>95C 900sec>(94C 15sec>50C 30sec>72C 30sec>72C 420sec)*50

Primer Name: SG012201	Type of Usage: Forward Primer	Tm: 71.5C at salt 1000.0 mM
Primer Name: SG012202	Type of Usage: Reverse Primer	Tm: 70C at salt 1000.0 mM
Primer Name: SG012203	Type of Usage: Forward Primer	Tm: 67.9C at salt 1000.0 mM
Primer Name: SG012204	Type of Usage: Reverse Primer	Tm: 70C at salt 1000.0 mM

Common Data ID: SG0123	Virus: SARS coronavirus
PMID: 15030680	Publication date:02/04	Type: RT-PCR
Temp: 50C 1800sec>95C 900sec>(95C 10sec>56C 10sec>72C 20sec)*50>72C 420sec

Primer Name: BNIoutS2	Type of Usage: Forward Primer	Tm: 70.2C at salt 1000.0 mM
Primer Name: BNIoutAs	Type of Usage: Reverse Primer	Tm: 74.7C at salt 1000.0 mM

Common Data ID: SG0128	Virus: SARS coronavirus
PMID: 14532176	Publication date:10/03	Type: PCR
Temp: 94C 600sec>(94C 30sec>50C 40sec>72C 15sec)*40>72C 600sec

Primer Name: coro3	Type of Usage: PCR	Tm: 52 C
Primer Name: coro4	Type of Usage: PCR	Tm: 48 C

Common Data ID: SG0129	Virus: SARS coronavirus
PMID: 14532176	Publication date:10/03	Type: RT-PCR
Temp: 45C 1800sec>95C 180sec>(95C 10sec>60C(-1C/cyc) 10sec>72C 30sec)10>(95C 10sec>56C 10sec>72C 30sec)40

Primer Name: BNIoutS	Type of Usage: Forward Primer	Tm: 70.2C at salt 1000.0 mM
Primer Name: BNoutAs	Type of Usage: Reverse Primer	Tm: 74.7C at salt 1000.0 mM

Common Data ID: SG0130	Virus: SARS coronavirus
PMID: 14532176	Publication date:10/03	Type: PCR
Temp: 95C 300sec>(95C 10sec>60C (-1C/cyc) 10sec>72C 20sec)10>(95C 10sec>56C 10sec>72C 20sec)20

Primer Name: BNIinS	Type of Usage: Forward Primer	Tm: 72C at salt 1000.0 mM
Primer Name: BNIAs	Type of Usage: Reverse Primer	Tm: 72.9C at salt 1000.0 mM

Common Data ID: SG0131	Virus: SARS coronavirus
PMID: 14662982	Publication date:12/03	Type: RT-PCR
Temp:

Primer Name: SG013101	Type of Usage: Forward Primer	Tm: 77.2C at salt 1000.0 mM
Primer Name: SG013102	Type of Usage: Reverse Primer	Tm: 80.6C at salt 1000.0 mM

Common Data ID: SG0144	Virus: SARS Coronavirus
PMID: 15131175	Publication date:05/05	Type: Q-RT-PCR
Temp: 95C 10sec>(95C 2sec>55C 12sec>72C10sec)*50>40C 30sec

Primer Name: BNITMSARS1	Type of Usage: PCR	Tm: 72 C at salt 1000.0mM
Primer Name: BNITMSARAs2	Type of Usage: PCR	Tm: 76.6 C at salt 1000.0mM
Primer Name: BNITMSARP	Type of Usage:	Tm: 77 C at salt 1000.0mM

Common Data ID: SG0145	Virus: SARS Coronavirus
PMID: 15131175	Publication date:05/05	Type: Q-RT-PCR
Temp: 95C 30sec>(95C 5sec>55C 15sec>72C 10sec)*45>40C 30sec

Primer Name: BNIoutS2	Type of Usage: PCR	Tm: 70.2 C at salt 1000.0mM
Primer Name: BNIouutAs	Type of Usage: PCR	Tm: 72.5 C at salt 1000.0mM

Common Data ID: SG0149	Virus: SARS coronavirus
PMID: 15131168	Publication date:05/04	Type: RT-PCR
Temp: 50C 600sec>95C 180sec>(95C 2sec>55C 12sec>72C 10sec)*50

Primer Name: SANS1	Type of Usage: Forward Primer	Tm: 72.5C at salt 1000.0 mM
Primer Name: SANP1	Type of Usage: PCR Probe	Tm: 77C at salt 1000.0 mM
Primer Name: SANPAs2	Type of Usage: Reverse Primer	Tm: 74.7C at salt 1000.0 mM

Common Data ID: SG0151	Virus: SARS coronavirus
PMID: 15243096	Publication date:07/04	Type: Multiplex PCR
Temp: 95C 180sec>(95C 60sec>50C 60sec>72C 60sec)*35

Primer Name: SpikeA	Type of Usage: Forward Primer	Tm: 54C
Primer Name: SpikeB	Type of Usage: Reverse Primer	Tm: 76.4C at salt 1000.0 mM
Primer Name: GeneMa	Type of Usage: Forward Primer	Tm: 76.4C at salt 1000.0 mM
Primer Name: GeneMb	Type of Usage: Reverse Primer	Tm: 74.4C at salt 1000.0 mM
Primer Name: GeneNa	Type of Usage: Forward Primer	Tm: 76.4C at salt 1000.0 mM
Primer Name: GeneNb	Type of Usage: Reverse Primer	Tm: 70.5C at salt 1000.0 mM

Common Data ID: SG0152	Virus: SARS coronavirus
PMID: 15031782	Publication date:04/04	Type: Nested RT-PCR
Temp:

Primer Name: SG015201	Type of Usage: Forward Primer	Tm: 71.5C at salt 1000.0 mM
Primer Name: SG015202	Type of Usage: Reverse Primer	Tm: 70C at salt 1000.0 mM
Primer Name: SG015203	Type of Usage: Forward Primer	Tm: 52C
Primer Name: SG015204	Type of Usage: Reverse Primer	Tm: 54C

Common Data ID: TA0050	Virus: SARS coronavirus
PMID: 15200815	Publication date:05/04	Type: RT-PCR
Temp: 94C 5min>(94C 45sec>60C 45sec>72C 45sec)*45>72C 7min

Primer Name: COR-1	Type of Usage: Forward Primer	Tm: 77.3C at salt 1000.0 mM
Primer Name: COR-2	Type of Usage: Reverse Primer	Tm: 72.6C at salt 1000.0 mM

Common Data ID: TA0051	Virus: SARS coronavirus
PMID: 15200815	Publication date:05/04	Type: RT-PCR
Temp: 94C 3min>(94C 0.75min>60C 0.75min>72C 0.75min)*45>72C 7min

Primer Name: COR-1	Type of Usage: Forward Primer	Tm: 77.3C at salt 1000.0 mM
Primer Name: COR-2	Type of Usage: Reverse Primer	Tm: 72.6C at salt 1000.0 mM

Common Data ID: TA0055	Virus: SARS coronavirus
PMID: 15349945	Publication date:09/04	Type: Multiplex RT-PCR
Temp: 94C 5min>(94C 0.5min>55C 0.5min>72C 0.5min)*30>72C 7min

Primer Name: TA005501	Type of Usage: Forward Primer	Tm: 72C at salt 1000.0 mM
Primer Name: TA005502	Type of Usage: Forward Primer	Tm: 72C at salt 1000.0 mM
Primer Name: TA005503	Type of Usage: Reverse Primer	Tm: 70C at salt 1000.0 mM
Primer Name: TA005504	Type of Usage: Reverse Primer	Tm: 70C at salt 1000.0 mM

Common Data ID: TA0056	Virus: SARS coronavirus
PMID: 15349945	Publication date:09/04	Type: Multiplex RT-PCR
Temp: 94C 5min>(94C 30sec>55C 30sec>72C 30sec)*30>72C 7min

Primer Name: TA005601	Type of Usage: Forward Primer	Tm: 72C at salt 1000.0 mM
Primer Name: TA005602	Type of Usage: Forward Primer	Tm: 72C at salt 1000.0 mM
Primer Name: TA005603	Type of Usage: Reverse Primer	Tm: 70C at salt 1000.0 mM
Primer Name: TA005604	Type of Usage: Reverse Primer	Tm: 70C at salt 1000.0 mM

Common Data ID: TA0059	Virus: SARS coronavirus
PMID: 15131154	Publication date:5/04	Type: RT-PCR
Temp: 94C 2min>(94C 30sec>54C 30sec>72C 30sec)*34>72C 10min

Primer Name: BNIout-sense	Type of Usage: Forward Primer	Tm: 70.2C at salt 1000.0 mM
Primer Name: BNIout-antisense	Type of Usage: Reverse Primer	Tm: 74.7C at salt 1000.0 mM

Common Data ID: TA0061	Virus: SARS coronavirus
PMID: 14633916	Publication date:11/03	Type: Real-time RT-PCR
Temp: 95C 5min>(94C 20sec>56C 1min)*40

Primer Name: SARSPol1 forward	Type of Usage: Forward Primer	Tm: 73.2C at salt 1000.0 mM
Primer Name: SARSPol1 reverse	Type of Usage: Reverse Primer	Tm: 71C at salt 1000.0 mM
Primer Name: SARSPol1 probe	Type of Usage: PCR Probe	Tm: 78.7C at salt 1000.0 mM
Primer Name: SARSN forward	Type of Usage: Forward Primer	Tm: 52C
Primer Name: SARSN reverse	Type of Usage: Reverse Primer	Tm: 72.5C at salt 1000.0 mM
Primer Name: SARSN probe	Type of Usage: PCR Probe	Tm: 75.3C at salt 1000.0 mM

Common Data ID: TA0062	Virus: SARS coronavirus
PMID: 15178653	Publication date:8/04	Type: Real-time RT-PCR
Temp:

Primer Name: SARSPol1 forward	Type of Usage: Forward Primer	Tm: 73.2C at salt 1000.0 mM
Primer Name: SARSPol1 reverse	Type of Usage: Reverse Primer	Tm: 71C at salt 1000.0 mM
Primer Name: SARSPol1 probe	Type of Usage: PCR Probe	Tm: 78.7C at salt 1000.0 mM
Primer Name: SARSN forward	Type of Usage: Forward Primer	Tm: 52C
Primer Name: SARSN reverse	Type of Usage: Reverse Primer	Tm: 72.5C at salt 1000.0 mM
Primer Name: SARSN probe	Type of Usage: PCR Probe	Tm: 75.3C at salt 1000.0 mM

Common Data ID: UM0012	Virus: SARS coronavirus
PMID: 12690092	Publication date:04/03	Type: RT-PCR
Temp: N/A

Primer Name: Cor-p-F2 (+)	Type of Usage: PCR	Tm: 68.5 C at salt 1000.0mM
Primer Name: Cor-p-F3 (+)	Type of Usage: PCR	Tm: 76.4 C at salt 1000.0mM
Primer Name: Cor-p-R1 (–)	Type of Usage:	Tm: 70.5 C at salt 1000.0mM

Common Data ID: UM0014	Virus: SARS coronavirus
PMID: 12711465	Publication date:04/03	Type: RT-PCR
Temp: 94C 8 min>(94C 1min>50C1min>72C 1min)*40

Primer Name: SARSF18	Type of Usage: PCR	Tm: 52 C
Primer Name: SARSR18	Type of Usage: PCR	Tm: 48 C

Common Data ID: UM0015	Virus: SARS Coronavirus
PMID: 12690091	Publication date:8/2003	Type: RT-PCR
Temp: 45C 30min>95C 3min(95C 10sec>60C 10sec[-1C/cyc]>72C 30sec)10>(95C 10sec>56C 10sec>72C 30sec)40

Primer Name: IN-2 (+)	Type of Usage:	Tm: 77 C at salt 1000.0mM
Primer Name: IN-4	Type of Usage:	Tm: 68.5 C at salt 1000.0mM

Common Data ID: UM0016	Virus: SARS Coronavirus
PMID: 12690091	Publication date:8/2003	Type: RT-PCR
Temp: 45C 30min>95C 3min(95C 10sec>60C 10sec[-1C/cyc]>72C 30sec)10>(95C 10sec>56C 10sec>72C 30sec)40

Primer Name: IN-6	Type of Usage: PCR	Tm: 77 C at salt 1000.0mM
Primer Name: IN-7	Type of Usage: PCR	Tm: 70.2 C at salt 1000.0mM

Common Data ID: UM0017	Virus: SARS Coronavirus
PMID: 12690091	Publication date:8/2003	Type: RT-PCR
Temp: 45C 30min>95C 3min(95C 10sec>60C 10sec[-1C/cyc]>72C 30sec)10>(95C 10sec>56C 10sec>72C 30sec)40

Primer Name: BNIoutS2	Type of Usage: PCR	Tm: 70.2 C at salt 1000.0mM
Primer Name: BNIouutAs	Type of Usage: PCR	Tm: 72.5 C at salt 1000.0mM

Common Data ID: UM0018	Virus: SARS Coronavirus
PMID: 12690091	Publication date:8/2003	Type: Nested PCR
Temp: 95C 3min>(95C 10sec>60C 10sec[-1C/cyc]>72C 20sec)10>(95C 10sec>56C 10sec>72C 20sec)20

Primer Name: SAR1S	Type of Usage: PCR	Tm: 74.4 C at salt 1000.0mM
Primer Name: SAR1As	Type of Usage: PCR	Tm: 74.4 C at salt 1000.0mM

Common Data ID: UM0019	Virus: SARS Coronavirus
PMID: 12690091	Publication date:8/2003	Type: Nested PCR
Temp: 95C 3min>(95C 10sec>60C 10sec[-1C/cyc]>72C 20sec)10>(95C 10sec>56C 10sec>72C 20sec)20

Primer Name: BNlinS	Type of Usage: PCR	Tm: 72 C at salt 1000.0mM
Primer Name: BNIinAs	Type of Usage: PCR	Tm: 72.9 C at salt 1000.0mM

Common Data ID: UM0020	Virus: SARS Coronavirus
PMID: 12690091	Publication date:8/2003	Type: Real-time PCR
Temp: 45C 15min>95C 3min>(95C 10sec>58C 30sec)*40

Primer Name: BNITMSARS1	Type of Usage: PCR	Tm: 72 C at salt 1000.0mM
Primer Name: BNITMSARAs2	Type of Usage: PCR	Tm: 76.6 C at salt 1000.0mM
Primer Name: BNITMSARP	Type of Usage:	Tm: 77 C at salt 1000.0mM

Common Data ID: UM0021	Virus: SARS coronavirus
PMID: N/A	Publication date:04/03	Type: RT-PCR
Temp: N/A

Primer Name: COR-1	Type of Usage: PCR	Tm: 77.3 C at salt 1000.0mM
Primer Name: COR-2	Type of Usage:	Tm: 72.6 C at salt 1000.0mM

Common Data ID: UM0022	Virus: SARS coronavirus
PMID: N/A	Publication date:04/03	Type: RT-PCR
Temp: N/A

Primer Name: HKUSense	Type of Usage: PCR	Tm: 52 C
Primer Name: HKUAntisense	Type of Usage: PCR	Tm: 48 C

Total 230 Oligos