A Simple Solution for Oligonucleotide Design



Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:

Total 256 Oligos

Common Data ID: BH0010 Virus: pestivirus type 1
PMID: 11376844Publication date:07/01 Type: RT-PCR
Temp: (95C 45sec>50C 60sec>72C 60sec)*35
Primer Name: E2f Type of Usage: Tm: Max Tm 70.5 C Min Tm 68.5 C at salt 1000.0mM
Primer Name: E2inf Type of Usage: Tm: 80.3 C at salt 1000.0mM
Primer Name: E2r Type of Usage: Tm: Max Tm 70 C Min Tm 67.9 C at salt 1000.0mM

Common Data ID: BH0045 Virus: Pestivirus type 1
PMID: 9491443Publication date:11/97 Type: RT-PCR
Temp: 94C 5min>(94C 1min>56C 1min>72C 1min)*35>72C 10min
Primer Name: PEST1 Type of Usage: Tm: 76.1 C at salt 1000.0mM
Primer Name: PEST2 Type of Usage: Tm: 72.5 C at salt 1000.0mM

Common Data ID: BH0049 Virus: Bovine viral diarrhea virus (isolate NADL), BVDV; Hog cholera virus (strain Brescia), HCV
PMID: 8593411Publication date:09/95 Type: RT-PCR
Temp: 94C 75sec>(94C 45sec>60C 60sec>72C 60sec)*35>72C 6min
Primer Name: P1 Type of Usage: Tm: 76.4 C at salt 1000.0mM
Primer Name: P3 Type of Usage: Tm: 77.2 C at salt 1000.0mM

Common Data ID: BH0057 Virus: Bovine viral diarrhea virus-2, BVDV2; Bovine viral diarrhea virus (isolate NADL), BVDV-NADL
PMID: 9850544Publication date:12/98 Type: RT-Nested PCR
Temp: 94C 2min>(94C 30sec>50C 45sec>72C 60sec)*30>72C 7min
Primer Name: PN00423 Type of Usage: Tm: 56 C
Primer Name: PN00424 Type of Usage: Tm: 54 C
Primer Name: PN00425 Type of Usage: Tm: 72 C at salt 1000.0mM
Primer Name: PN00426 Type of Usage: Tm: 72 C at salt 1000.0mM

Common Data ID: BM0039 Virus: pestivirus type 1
PMID: 9088554Publication date:12/96 Type: RT-Nested PCR
Temp: (94C 1min>56C 2min>72C 2min)*30
Primer Name: PF1 Type of Usage: Tm: Max Tm 74.1 C Min Tm 72 C at salt 1000.0mM
Primer Name: PR1 Type of Usage: Tm: 72 C at salt 1000.0mM
Primer Name: PF2 Type of Usage: Tm: Max Tm 56 C Min Tm 54 C
Primer Name: PR2 Type of Usage: Tm: 56 C

Common Data ID: BM0049 Virus: pestivirus type 1, BVDV
PMID: 10590128Publication date:01/00 Type: RT-PCR
Temp: (94C 30sec>54C 30sec>72C 30sec)*30
Primer Name: BVDV 56 Type of Usage: Tm: 54 C
Primer Name: BVDV 57 Type of Usage: Tm: 76.4 C at salt 1000.0mM

Common Data ID: BM0050 Virus: Border disease virus strain Moredun cp, BDV cp; Border disease virus strain Moredun ncp, BDV ncp; Hog cholera virus (strain Brescia), CSFV; bovine viral diarrhea virus (isolate NADL), BVDV NADL
PMID: 9129644Publication date:04/97 Type: RT-PCR
Temp: (94C 1min>56C 1min>72C 1min)*35>72C 7min
Primer Name: 324 Type of Usage: Tm: 73.9 C at salt 1000.0mM
Primer Name: 326 Type of Usage: Tm: 72.5 C at salt 1000.0mM

Common Data ID: BM0051 Virus: Border disease virus strain Moredun cp, BDV cp; Border disease virus strain Moredun ncp, BDV ncp; Hog cholera virus (strain Brescia), CSFV; bovine viral diarrhea virus (isolate NADL), BVDV NADL
PMID: 9129644Publication date:04/97 Type: RT-PCR
Temp: (94C 1min>52C 1min>72C 1min)*35>72C 7min
Primer Name: BD1 Type of Usage: Tm: 72.5 C at salt 1000.0mM
Primer Name: BD2 Type of Usage: Tm: Max Tm 74.1 C Min Tm 67.9 C at salt 1000.0mM

Common Data ID: BM0055 Virus: bovine viral diarrhea virus (isolate NADL), NADL BVDV
PMID: 9210942Publication date:1991 Type: RT-PCR
Temp: 94C 2min>(94C 1min>55C 1.5min>72C 3min)*30>72C 7min
Primer Name: 6322 Type of Usage: Tm: 77 C at salt 1000.0mM
Primer Name: 7451 Type of Usage: Tm: 80.4 C at salt 1000.0mM

Common Data ID: BM0471 Virus: bovine viral diarrhea virus (isolate NADL); bovine viral diarrhea virus strain Oregon C24V; pestivirus type 1 (isolate 890)
PMID: 11524161Publication date:10/01 Type: Real Time RT-PCR
Temp: 48C 30min>95C 10min>(95C 15sec>60C 60sec)*45
Primer Name: PD00256 Type of Usage: Tm: 76.4 C at salt 1000.0mM
Primer Name: PD00257 Type of Usage: Tm: 74.4 C at salt 1000.0mM
Primer Name: PD00258 Type of Usage: Tm: 79 C at salt 1000.0mM
Primer Name: PD00259 Type of Usage: Tm: 80.5 C at salt 1000.0mM

Common Data ID: BM0559 Virus: pestivirus Giraffe-1
PMID: 11145272Publication date:2000 Type: RT-PCR
Temp: (94C 30sec>55C 120sec>72C 120sec)*30
Primer Name: R1 Type of Usage: Tm: 72 C at salt 1000.0mM
Primer Name: F1 Type of Usage: Tm: Max Tm 74.1 C Min Tm 72 C at salt 1000.0mM

Common Data ID: BM1351 Virus: pestivirus type 1; Bovine viral diarrhea virus genotype 2
PMID: 11939332Publication date:03/02 Type: RT-PCR
Temp: 50C 30min>95C 15min>(94C 1min>55C 1min>72C 2min)*40>72C 7min
Primer Name: PB00933 Type of Usage: Tm: Max Tm 78.2 C Min Tm 74.1 C at salt 1000.0mM
Primer Name: PB00934 Type of Usage: Tm: 74.5 C at salt 1000.0mM
Primer Name: PB00935 Type of Usage: Tm: Max Tm 82.3 C Min Tm 78.2 C at salt 1000.0mM

Common Data ID: BM1806 Virus: pestivirus type 1
PMID: 11878878Publication date:12/01 Type: RT-Nested PCR 1(1)
Temp: (94C 30sec>55C 30sec>72C 60sec)*35>68C 10min
Primer Name: Ol-B22.1R Type of Usage: PCRTm: 70.5 C at salt 1000.0mM
Primer Name: Ol-BVDV32 Type of Usage: Tm: 75.5 C at salt 1000.0mM
Primer Name: Ol-B32II Type of Usage: Tm: 78.3 C at salt 1000.0mM
Primer Name: Ol-JasIns1 Type of Usage: Tm: 54 C
Primer Name: Ol-JasIns2 Type of Usage: Tm: 50 C

Common Data ID: BM1807 Virus: pestivirus type 1
PMID: 11878878Publication date:12/01 Type: RT-Nested PCR 1(2)
Temp: (94C 30sec>55C 30sec>72C 60sec)*35>68C 10min
Primer Name: Ol-B19II Type of Usage: Tm: 67.9 C at salt 1000.0mM
Primer Name: Ol-B22.1R Type of Usage: Tm: 70.5 C at salt 1000.0mM

Common Data ID: BM1808 Virus: pestivirus type 1
PMID: 11878878Publication date:12/01 Type: RT-Nested PCR 1(3)
Temp: (94C 30sec>52C 30sec>68C 90sec)*9>(94C 30sec>52C 30sec>68C 45sec[+5sec/cyc])*25>68C 7min
Primer Name: Ol-B22.1R Type of Usage: Tm: 70.5 C at salt 1000.0mM
Primer Name: Ol-BVDV32 Type of Usage: Tm: 75.5 C at salt 1000.0mM
Primer Name: Ol-B32II Type of Usage: Tm: 78.3 C at salt 1000.0mM
Primer Name: Ol-JasIns1 Type of Usage: Tm: 54 C
Primer Name: Ol-JasIns2 Type of Usage: Tm: 50 C

Common Data ID: BM1809 Virus: pestivirus type 1
PMID: 11878878Publication date:12/01 Type: RT-Nested PCR 1(4)
Temp: (94C 30sec>52C 30sec>68C 90sec)*9>(94C 30sec>52C 30sec>68C 45sec[+5sec/cyc])*25>68C 7min
Primer Name: Ol-B19II Type of Usage: Tm: 67.9 C at salt 1000.0mM
Primer Name: Ol-B22.1R Type of Usage: Tm: 70.5 C at salt 1000.0mM

Common Data ID: BM1810 Virus: pestivirus type 1
PMID: 11878878Publication date:12/01 Type: RT-Nested PCR 1(5)
Temp: 50C 30min>95C 15min>(94C 30sec>53C 30sec>72C 60sec)*39
Primer Name: Ol-B22.1R Type of Usage: Tm: 70.5 C at salt 1000.0mM
Primer Name: Ol-JasIns2 Type of Usage: Tm: 50 C

Common Data ID: BM1811 Virus: pestivirus type 1
PMID: 11878878Publication date:12/01 Type: RT-Nested PCR 2
Temp: 95C 2min>(95C 40sec>56C 40sec>72C 50sec)*30>72C 5min
Primer Name: Ol-LSIV Type of Usage: Tm: 83.4 C at salt 1000.0mM
Primer Name: Ol-Jas/Jf Type of Usage: Tm: 72.5 C at salt 1000.0mM

Common Data ID: CW0027 Virus: bovine viral diarrhea virus, BVDV, Border Disease Virus, BDV
PMID: 1311133Publication date:01/92 Type: RT-PCR
Temp: (94C 1min>60C 2min>72C 4min)*50
Primer Name: 147R Type of Usage: PCRTm: 89.1 C at salt 1000.0mM
Primer Name: 180L Type of Usage: PCRTm: 87 C at salt 1000.0mM
Primer Name: 145 Type of Usage: Hydridization ProbeTm: 88 C at salt 1000.0mM

Common Data ID: CW0034 Virus: Bovine viral diarrhea virus, BVDV
PMID: 1318605Publication date:07/92 Type: RT-PCR
Temp: 94C 5min>(94C 1min>50C 1min>72C 1min)*30
Primer Name: 10 Type of Usage: PCRTm: 44 C
Primer Name: 14 Type of Usage: PCRTm: 65.8 C at salt 1000.0mM
Primer Name: 11 Type of Usage: OtherTm: 52 C

Common Data ID: HI0005 Virus: bovine viral diarrhea virus (isolate NADL); pestivirus type 2 strain BVDV890; border disease virus strain BD31
PMID: 8578861Publication date:10/95 Type: RT-Nested PCR 1
Temp: 50C 30min>94C 2min>(94C 1min>55C 1min>72C 1min)*30>72C 10min
Primer Name: P1 Type of Usage: PCRTm: 75.5 C at salt 1000.0mM
Primer Name: P2 Type of Usage: PCRTm: 77.8 C at salt 1000.0mM

Common Data ID: HI0006 Virus: bovine viral diarrhea virus (isolate NADL); pestivirus type 2 strain BVDV890; Border disease virus strain BD31
PMID: 8578861Publication date:10/95 Type: RT-Nested PCR 2
Temp: (94C 60sec>50C 45sec>72C 45sec)*25
Primer Name: TS1 Type of Usage: PCRTm: 75.6 C at salt 1000.0mM
Primer Name: TS2 Type of Usage: PCRTm: 70 C at salt 1000.0mM
Primer Name: TS3 Type of Usage: PCRTm: 78 C at salt 1000.0mM
Primer Name: P2 Type of Usage: PCRTm: 77.8 C at salt 1000.0mM

Common Data ID: JP0040 Virus: Bovine viral diarrhea virus-1, BVDV-1; Bovine viral diarrhea virus-2, BVDV-2
PMID: 11808702Publication date:01/02 Type: RT-Semi-Nested PCR 1
Temp: 94C 2min>(94C 1min>55C 1min>72C 1min)*30>72C 10min.
Primer Name: P1 Type of Usage: Tm: 75.5 C at salt 1000.0mM
Primer Name: P2 Type of Usage: Tm: 77.8 C at salt 1000.0mM

Common Data ID: JP0041 Virus: bovine viral diarrhea virus type 2, BVDV 2
PMID: 11808702Publication date:01/02 Type: RT-Semi-Nested PCR 2
Temp: 94C 2min>(94C 1min>50C 1min>72C 1min)*30>72C 10min
Primer Name: P2 Type of Usage: Tm: 77.8 C at salt 1000.0mM
Primer Name: TS2 Type of Usage: Tm: 70 C at salt 1000.0mM

Common Data ID: KS0014 Virus: pestivirus type 1, BVDV
PMID: 10028179Publication date:01/99 Type: RT-Nested PCR 1
Temp: 94C 3min>(94C 45sec>54C 60sec>72C 60sec)*35 >72C 7min
Primer Name: P1U Type of Usage: PCRTm: 76.4 C at salt 1000.0mM
Primer Name: Pest2-L Type of Usage: PCRTm: 72.5 C at salt 1000.0mM

Common Data ID: KS0015 Virus: pestivirus type 1, BVDV
PMID: 10028179Publication date:01/99 Type: RT-Nested PCR 2
Temp: (94C 10sec>50C 30sec>72C 30sec)*25
Primer Name: 324-U Type of Usage: PCRTm: 73.9 C at salt 1000.0mM
Primer Name: Pest2-L Type of Usage: PCRTm: 72.5 C at salt 1000.0mM

Common Data ID: KS0025 Virus: pestivirus type 1, BVDV
PMID: 8408445Publication date:08/93 Type: RT-Nested PCR 1
Temp: 94C 30sec>(94C 30sec>55C 45sec[+2sec/cyc]>72C 30sec[+5sec/cyc])*25>72C 10min
Primer Name: A Type of Usage: PCRTm: 69.7 C at salt 1000.0mM
Primer Name: D Type of Usage: PCRTm: 74.7 C at salt 1000.0mM

Common Data ID: KS0026 Virus: pestivirus type 1, BVDV
PMID: 8408445Publication date:08/93 Type: RT-Nested PCR 2
Temp: 94C 30sec>(94C 30sec>55C 45sec[+2sec/cyc]>72C 30sec[+5sec/cyc])*30>72C 10min
Primer Name: B Type of Usage: PCRTm: 69.7 C at salt 1000.0mM
Primer Name: C Type of Usage: PCRTm: 54 C
Primer Name: E Type of Usage: HybridizationTm: 74.7 C at salt 1000.0mM

Common Data ID: MO0004 Virus: pestivirus type 1 BVDV
PMID: 10028170Publication date:01/99 Type: RT-PCR
Temp: 95C 5min>(94C 1min>57C 1min>72C 1min)*35
Primer Name: B1 Type of Usage: PCRTm: 76.4 C at salt 1000.0mM
Primer Name: BE Type of Usage: PCRTm: 74.4 C at salt 1000.0mM
Primer Name: B2 Type of Usage: PCRTm: 72.5 C at salt 1000.0mM

Common Data ID: MO0005 Virus: bovine viral diarrhea virus, BVDV-1, BVDV-2
PMID: 10028170Publication date:01/99 Type: RT-PCR
Temp: 95C 5min>(94C 1min>52C 1min>72C 1min)*35
Primer Name: B3 Type of Usage: PCRTm: 54 C
Primer Name: B4 Type of Usage: PCRTm: 56 C
Primer Name: B5 Type of Usage: PCRTm: 56 C
Primer Name: B6 Type of Usage: PCRTm: 56 C

Common Data ID: MP0044 Virus: bovine viral diarrhea virus, BVDV
PMID: 10730955Publication date:03/00 Type: RT-PCR
Temp: 95C 4min>(95C 1min>56C 1.5min>74C 1min)*40
Primer Name: UTR1 Type of Usage: PCRTm: 58 C
Primer Name: UTR2 Type of Usage: PCRTm: 58 C
Primer Name: 324 Type of Usage: PCRTm: 73.9 C at salt 1000.0mM
Primer Name: 326 Type of Usage: PCRTm: 72.5 C at salt 1000.0mM

Common Data ID: MP0053 Virus: bovine viral diarrhea virus (isolate NADL), BVDV
PMID: 7714180Publication date:02/95 Type: RT-PCR
Temp: 42C 30min>95C 3min>(95C 30sec>58C 15sec>72C 30sec)*35>72C 10min
Primer Name: BVD-99 Type of Usage: PCRTm: 74.1 C at salt 1000.0mM
Primer Name: BVD-342 Type of Usage: PCRTm: 73.7 C at salt 1000.0mM

Common Data ID: MP0070 Virus: bovine viral diarrhea virus (isolate NADL), BVDV
PMID: 7524039Publication date:10/94 Type: RT-PCR
Temp: (94C 30sec>56C 1min>74C 1min)*30
Primer Name: MP00701 Type of Usage: PCRTm: 72 C at salt 1000.0mM
Primer Name: MP00702 Type of Usage: PCRTm: 70 C at salt 1000.0mM

Common Data ID: MP0071 Virus: bovine viral diarrhea virus (isolate NADL), BVDV
PMID: 1322931Publication date:07/92 Type: RT-PCR
Temp: 94C 2min>(94C 1min>55C 1.5min>72C 3min)*30>72C 7min
Primer Name: 12434 Type of Usage: PCRTm: 60 C
Primer Name: 231 Type of Usage: PCRTm: 78 C at salt 1000.0mM

Common Data ID: MP0074 Virus: bovine viral diarrhea virus, BVDV
PMID: 8068309Publication date:03/94 Type: RT-PCR
Temp: (95C 1min>55C 1min> 75C 2min)*40
Primer Name: Primer 2.1 Type of Usage: PCRTm: 65.8 C at salt 1000.0mM
Primer Name: Primer 2.2 Type of Usage: PCRTm: 65.8 C at salt 1000.0mM

Common Data ID: MP0075 Virus: bovine viral diarrhea virus (isolate NADL), BVDV
PMID: 2162162Publication date:12/89 Type: RT-PCR
Temp: 95C 3min>62C 5min>(95C 30sec>62C 45sec)*34>62C 10min
Primer Name: PBVD4 Type of Usage: PCRTm: 73.2 C at salt 1000.0mM
Primer Name: PBVD3 Type of Usage: PCRTm: 73.2 C at salt 1000.0mM
Primer Name: PBVD7 Type of Usage: PCRTm: 76.1 C at salt 1000.0mM
Primer Name: PBVD8 Type of Usage: PCRTm: 76.1 C at salt 1000.0mM
Primer Name: PBVD9 Type of Usage:
Tm: 67.9 C at salt 1000.0mM
Primer Name: PBVD10 Type of Usage: PCRTm: 70 C at salt 1000.0mM

Common Data ID: MP0076 Virus: bovine viral diarrhea virus (isolate NADL), BVDV
PMID: 8384762Publication date:01/93 Type: RT-PCR
Temp: (95C 1min>53C 1min>72C 2min)*35
Primer Name: B Type of Usage: PCRTm: 74.1 C at salt 1000.0mM
Primer Name: C Type of Usage: PCRTm: 54 C

Common Data ID: MP0077 Virus: bovine viral diarrhea virus (isolate NADL), BVDV
PMID: 2164736Publication date:04/90 Type: RT-PCR
Temp: see additional note
Primer Name: A Type of Usage: PCRTm: 79.3 C at salt 1000.0mM
Primer Name: B Type of Usage: PCRTm: 82.1 C at salt 1000.0mM

Common Data ID: MP0079 Virus: pestivirus type 3, BDV
PMID: 7985409Publication date:04/94 Type: RT-PCR
Temp: 94C 2min>(94C 1min>55C 1min>72C 3min)*30>72C 30min
Primer Name: p1 Type of Usage: PCRTm: 74.7 C at salt 1000.0mM
Primer Name: p2 Type of Usage: PCRTm: 74.7 C at salt 1000.0mM
Primer Name: p3 Type of Usage: PCRTm: 85.2 C at salt 1000.0mM
Primer Name: p4 Type of Usage: PCRTm: 74.7 C at salt 1000.0mM
Primer Name: p5 Type of Usage: PCRTm: 80.5 C at salt 1000.0mM
Primer Name: p6 Type of Usage: PCRTm: 80.6 C at salt 1000.0mM

Common Data ID: MP0080 Virus: bovine viral diarrhea virus (isolate NADL), BVDV
PMID: 1656820Publication date:08/91 Type: RT-PCR
Temp: 94C 1min>37C 30sec>72C 10min>(94C 1min>37C 30sec>72C 30sec)*5>(94C 1min>55C 30sec>72C 30sec)*30
Primer Name: p80.1 Type of Usage: PCRTm: Max Tm 85.1 C Min Tm 79 C at salt 1000.0mM
Primer Name: p80.2 Type of Usage: PCRTm: Max Tm 82 C Min Tm 76.2 C at salt 1000.0mM

Common Data ID: MP0081 Virus: bovine viral diarrhea virus (isolate NADL), BVDV
PMID: 8011781Publication date:01/94 Type: RT-PCR
Temp: (95C 1min>55C 1min>72C 2min)*40
Primer Name: MP00811 Type of Usage: PCRTm: 65.8 C at salt 1000.0mM
Primer Name: MP00812 Type of Usage: PCRTm: 65.8 C at salt 1000.0mM

Common Data ID: MP0082 Virus: bovine viral diarrhea virus (isolate NADL), BVDV
PMID: 8011781Publication date:01/94 Type: RT-PCR
Temp: (95C 1min>55C 1min>72C 2min)*40
Primer Name: MP00821 Type of Usage: PCRTm: 56 C
Primer Name: MP00822 Type of Usage: PCRTm: 54 C

Common Data ID: MP0083 Virus: bovine viral diarrhea virus (isolate NADL), BVDV
PMID: 1850232Publication date:1991 Type: Hybridization
Temp: 50C 2hr
Primer Name: ND001 Type of Usage: HybridizationTm: 72 C at salt 1000.0mM
Primer Name: ND002 Type of Usage: HybridizationTm: 70 C at salt 1000.0mM
Primer Name: ND003 Type of Usage: HybridizationTm: 67.9 C at salt 1000.0mM
Primer Name: ND004 Type of Usage: HybridizationTm: 70 C at salt 1000.0mM
Primer Name: ND005 Type of Usage: HybridizationTm: 72 C at salt 1000.0mM
Primer Name: ND006 Type of Usage: HybridizationTm: 67.9 C at salt 1000.0mM

Common Data ID: MP0084 Virus: bovine viral diarrhea virus type 2, BVDV-2
PMID: 9633045Publication date:02/98 Type: Nested RT-PCR
Temp: (94C 10sec>50C 15sec>72C 30sec)*30>72C 10min
Primer Name: MP00841 Type of Usage: PCRTm: 52 C
Primer Name: MP00842 Type of Usage: PCRTm: 52 C
Primer Name: MP00843 Type of Usage: PCRTm: 72 C at salt 1000.0mM
Primer Name: MP00844 Type of Usage: PCRTm: 72 C at salt 1000.0mM

Common Data ID: MP0085 Virus: Bovine viral diarrhea virus-1, BVDV-1
PMID: 9633045Publication date:02/98 Type: Nested RT-PCR
Temp: (94C 10sec>50C 15sec>72C 30sec)*30>72C 10min
Primer Name: MP00851 Type of Usage: PCRTm: 72.5 C at salt 1000.0mM
Primer Name: MP00852 Type of Usage: PCRTm: 46 C
Primer Name: MP00853 Type of Usage: PCRTm: 58 C

Common Data ID: MP0086 Virus: bovine viral diarrhea virus, BVDV
PMID: 8074863Publication date:06/94 Type: RT-PCR
Temp: (95C 1min>60C 1min)*40
Primer Name: P1 Type of Usage: PCRTm: 72.5 C at salt 1000.0mM
Primer Name: P2 Type of Usage: PCRTm: 72.5 C at salt 1000.0mM

Common Data ID: MP0087 Virus: bovine viral diarrhea virus (isolate NADL), BVDV NADL
PMID: 8264668Publication date:10/93 Type: RT-PCR
Temp: 95C 4min 30sec>(95C 30sec>50C 30sec>72C 30sec)*36
Primer Name: 10 Type of Usage: PCRTm: 74.1 C at salt 1000.0mM
Primer Name: 20 Type of Usage: PCRTm: 52 C

Common Data ID: MP0089 Virus: bovine viral diarrhea virus (isolate NADL); bovine viral diarrhea virus strain Oregon C24V; bovine viral diarrhea virus (strain SD-1); Bovine viral diarrhea virus genotype 2, BVDV II
PMID: 7975238Publication date:11/94 Type: RT-Nested PCR 1
Temp: 56C 1hr>94C 4min>(94C 10sec>50C 45sec>72C 45sec)*25>72C 10min
Primer Name: MP00891 Type of Usage: PCRTm: 56 C
Primer Name: MP00892 Type of Usage: PCRTm: 54 C

Common Data ID: MP0110 Virus: bovine viral diarrhea virus (isolate NADL), BVDV
PMID: 7597858Publication date:10/94 Type: RT-PCR
Temp: 94C 45sec>(94C 45sec>55C 45sec[+2sec/cyc]>72C 30sec[+5sec/cyc])*35>72C 10min
Primer Name: MP01101 Type of Usage: PCRTm: 69.7 C at salt 1000.0mM
Primer Name: MP01102 Type of Usage: PCRTm: 54 C

Common Data ID: MP0111 Virus: bovine viral diarrhea virus (isolate NADL), BVDV
PMID: 7597858Publication date:10/94 Type: RT-Nested PCR 1
Temp: 94C 45sec>(94C 45sec>55C 45sec[+2sec/cyc]>72C 30sec[+5sec/cyc])*25>72C 10min
Primer Name: MP01111 Type of Usage: PCRTm: 69.7 C at salt 1000.0mM
Primer Name: MP01112 Type of Usage: PCRTm: 74.7 C at salt 1000.0mM

Common Data ID: MP0112 Virus: bovine viral diarrhea virus (isolate NADL), BVDV
PMID: 7597858Publication date:10/94 Type: RT-Nested PCR 2
Temp: 94C 45sec>(94C 45sec>55C 45sec[+2sec/cyc]>72C 30sec[+5sec/cyc])*30>72C 10min
Primer Name: MP01101 Type of Usage: Tm: 69.7 C at salt 1000.0mM
Primer Name: MP01102 Type of Usage: Tm: 54 C

Common Data ID: MP0142 Virus: bovine viral diarrhea virus (isolate NADL), BVDV
PMID: 10403696Publication date:1999 Type: RT-PCR
Temp: (94C 1min>56C 1min>72C 1min)*35
Primer Name: B11 Type of Usage: PCRTm: Max Tm 74.7 C Min Tm 73.3 C at salt 1000.0mM
Primer Name: B12 Type of Usage: PCRTm: Max Tm 76.1 C Min Tm 74.1 C at salt 1000.0mM

Common Data ID: MP0143 Virus: bovine viral diarrhea virus, BVDV
PMID: 10403696Publication date:1999 Type: RT-PCR
Temp: (94C 1min>56C 1min>72C 1min)*35
Primer Name: B21 Type of Usage: PCRTm: 76.6 C at salt 1000.0mM
Primer Name: B22 Type of Usage: PCRTm: 74.7 C at salt 1000.0mM

Common Data ID: MP0152 Virus: Bovine viral diarrhea virus, BVDV
PMID: 9787488Publication date:02/98 Type: RT-Nested PCR 1
Temp: (94C 45sec>50C 30sec>72C 60sec)*5>(94C 45sec>45C 60sec>72C 180sec)*30>72C 7min
Primer Name: OPES 13a Type of Usage: Tm: 76.6 C at salt 1000.0mM
Primer Name: OPES 114a Type of Usage: Tm: 75.5 C at salt 1000.0mM

Common Data ID: MP0153 Virus: Bovine viral diarrhea virus, BVDV
PMID: 9787488Publication date:02/98 Type: RT-Nested PCR 2
Temp: (94C 45sec>55C 30sec>72C 60sec)*5>(94C 45sec>50C 60sec>72C 180sec)*30>72C 7min
Primer Name: OPES 11 Type of Usage: Tm: 82 C at salt 1000.0mM
Primer Name: OPES 12a Type of Usage: Tm: 78.5 C at salt 1000.0mM

Common Data ID: MP0257 Virus: bovine viral diarrhea virus (strain SD-1), BVDV
PMID: 11131328Publication date:10/00 Type: RT-Nested PCR 1
Temp: 37C 45min>95C 5min>(94C 1min>55C 1min>72C 1min)*20>72C 10min
Primer Name: BVD 100 Type of Usage: Tm: 58 C
Primer Name: HCV 368 Type of Usage: Tm: 52 C

Common Data ID: MP0258 Virus: bovine viral diarrhea virus (strain SD-1), BVDV
PMID: 11131328Publication date:10/00 Type: RT- Nested PCR 2
Temp: (94C 1min>55C 1min>72C 45 sec)*30>72C 10min
Primer Name: HCV 368 Type of Usage: Tm: 52 C
Primer Name: BVD 180 Type of Usage: Tm: Max Tm 78.5 C Min Tm 76.6 C at salt 1000.0mM

Common Data ID: MP0264 Virus: bovine viral diarrhea virus (isolate NADL), BVDV
PMID: 9129644Publication date:04/97 Type: RT-PCR
Temp: (94C 1min>56C 1min>72C 1min)*35>72C 7min
Primer Name: primer 324 Type of Usage: Tm: 73.9 C at salt 1000.0mM
Primer Name: primer 326 Type of Usage: Tm: 72.5 C at salt 1000.0mM

Common Data ID: MP0265 Virus: bovine viral diarrhea virus (isolate NADL), BVDV
PMID: 9129644Publication date:04/97 Type: RT-PCR
Temp: (94C 1min>52C 1min>72C 1min)*35>72C 7min
Primer Name: BD1 Type of Usage: PCRTm: 74.7 C at salt 1000.0mM
Primer Name: BD2 Type of Usage: PCRTm: Max Tm 74.1 C Min Tm 67.9 C at salt 1000.0mM

Common Data ID: MP0299 Virus: bovine viral diarrhea virus (isolate NADL), BVDV
PMID: 10325368Publication date:06/99 Type: RT-Nested Multiplex PCR 1
Temp: (94C 20sec>50C 30sec>72C 30sec)*25>72C 15min
Primer Name: PD00079 Type of Usage: Tm: 72 C at salt 1000.0mM
Primer Name: PD00080 Type of Usage: Tm: Max Tm 72 C Min Tm 70 C at salt 1000.0mM

Common Data ID: MP0865 Virus: Bovine viral diarrhea virus (isolate NADL), BVDV
PMID: 12421586Publication date:12/02 Type: RT-Semi-nested PCR
Temp: 95C 3min>(95C 30sec>55C 30sec>72C 60sec)*25>72C 3min
Primer Name: BVD43 Type of Usage: Tm: 72.5 C at salt 1000.0mM
Primer Name: BVDu1 Type of Usage: Tm: Max Tm 78.5 C Min Tm 74.7 C at salt 1000.0mM
Primer Name: BVDu2 Type of Usage: Tm: Max Tm 58 C Min Tm 52 C

Common Data ID: MP0866 Virus: Bovine viral diarrhea virus, BVDV
PMID: 12421586Publication date:12/02 Type: RT-PCR
Temp: 95C 30sec>(95C 1sec>55C 15sec>72C 15sec)*45
Primer Name: BVDs1 Type of Usage: Tm: 76.8 C at salt 1000.0mM
Primer Name: BVDs2 Type of Usage: Tm: 77.3 C at salt 1000.0mM
Primer Name: BVDl1 Type of Usage: Tm: 70.5 C at salt 1000.0mM
Primer Name: BVDu3 Type of Usage: Tm: 70 C at salt 1000.0mM

Common Data ID: SK0002 Virus: Bovine viral diarrhea virus (strain SD-1), BVDV strain SD-1
PMID: 7667908Publication date:04/95 Type: RT-PCR
Temp: 94C 4min>(94C 1min>55C 1.5min>72C 3min)*30>72C 7min
Primer Name: p80 2 Type of Usage: Tm: 80.4 C at salt 1000.0mM
Primer Name: p80 1 Type of Usage: Tm: 77 C at salt 1000.0mM
Primer Name: 5'UTR 1 Type of Usage: Tm: 58 C
Primer Name: 5'UTR 2 Type of Usage: Tm: 58 C

Common Data ID: SK0060 Virus: Bovine viral diarrhea virus, BVDV
PMID: 11131328Publication date:10/00 Type: RT-Nested PCR
Temp: (94C 60sec>55C 60sec>72C 45sec)*30>72C 10min
Primer Name: BVD100 Type of Usage: Tm: 58 C
Primer Name: HCV368 Type of Usage: Tm: 52 C
Primer Name: BVD180 Type of Usage: Tm: Max Tm 78.5 C Min Tm 76.6 C at salt 1000.0mM

Common Data ID: SK0064 Virus: bovine viral diarrhea virus (isolate NADL), NADL; pestivirus type 1 strain CS8644, CS8644
PMID: 11085469Publication date:11/00 Type: Universal RT-PCR
Temp: 50C 45min>94C 2min>50C 4min>72C 5min>(94C 0.5min>50C 2min>72C 5min)*35>72C 15min
Primer Name: BVD324 Type of Usage: Tm: 73.9 C at salt 1000.0mM
Primer Name: BVD326 Type of Usage: Tm: 72.5 C at salt 1000.0mM

Common Data ID: SK0065 Virus: Pestivirus type 1
PMID: 11390104Publication date:08/01 Type: RT-PCR
Temp: 60C 30min>94C 1min>(94C 15sec>63C 40sec)*40>63C 7min
Primer Name: PN00285 Type of Usage: Tm: 73.9 C at salt 1000.0mM
Primer Name: PN00286 Type of Usage: Tm: 72.5 C at salt 1000.0mM

Common Data ID: SK0102 Virus: pestivirus type 1, BVDV1; Bovine viral diarrhea virus genotype 2, BVDV2
PMID: 10325368Publication date:06/99 Type: RT-Nested PCR
Temp: (94C 20sec>50C 30sec>72C 30sec)*40>72C 15min
Primer Name: PN00391 Type of Usage: Tm: Max Tm 72 C Min Tm 70 C at salt 1000.0mM
Primer Name: PN00392 Type of Usage: Tm: Max Tm 72 C Min Tm 70 C at salt 1000.0mM
Primer Name: PN00393 Type of Usage: Tm: 71 C at salt 1000.0mM
Primer Name: PN00394 Type of Usage: Tm: 67.9 C at salt 1000.0mM
Primer Name: PN00395 Type of Usage: Tm: Max Tm 72.9 C Min Tm 71 C at salt 1000.0mM

Common Data ID: SK0111 Virus: Classical swine fever virus Alfort/187, CSFV-Alfort/187; bovine viral diarrhea virus isolate NADL, BVDV-NADL; Hog cholera virus (strain Brescia), CSFV-Brescia
PMID: 11377717Publication date:06/01 Type: RT-PCR
Temp: 60C 60min>94C 3min>(94C 30sec>60C 60sec)*40>60C 7min
Primer Name: pest104F Type of Usage: Tm: 78.7 C at salt 1000.0mM
Primer Name: pest402R Type of Usage: Tm: 77 C at salt 1000.0mM
Primer Name: csfvpr-2 Type of Usage: Tm: Max Tm 77 C Min Tm 73.6 C at salt 1000.0mM
Primer Name: pestpr-2 Type of Usage: Tm: Max Tm 78.5 C Min Tm 74.7 C at salt 1000.0mM

Common Data ID: SK0112 Virus: Classical swine fever virus Alfort/187, CSFV-Alfort/187; bovine viral diarrhea virus isolate NADL, BVDV-NADL; Hog cholera virus (strain Brescia), CSFV-Brescia
PMID: 11377717Publication date:06/01 Type: RT-PCR
Temp: 48C 45min>94C 2min>(94C 30sec>60C 60sec>68C 120sec)*40>68C 7min
Primer Name: panpest-3F Type of Usage: Tm: 78.8 C at salt 1000.0mM
Primer Name: panpest-4R Type of Usage: Tm: Max Tm 77.2 C Min Tm 72.3 C at salt 1000.0mM
Primer Name: bvdvpr-1 Type of Usage: Tm: Max Tm 87.3 C Min Tm 79.3 C at salt 1000.0mM
Primer Name: csfvpr-1 Type of Usage: Tm: Max Tm 80.6 C Min Tm 74.7 C at salt 1000.0mM
Primer Name: pest104F Type of Usage: Tm: 78.7 C at salt 1000.0mM
Primer Name: pest402R Type of Usage: Tm: 77 C at salt 1000.0mM
Primer Name: csfvpr-2 Type of Usage: Tm: Max Tm 77 C Min Tm 73.6 C at salt 1000.0mM
Primer Name: pestpr-2 Type of Usage: Tm: Max Tm 78.5 C Min Tm 74.7 C at salt 1000.0mM

Common Data ID: SK0116 Virus: pestivirus type 1 WI-38(CCL75); pestivirus type 2 CPA(CCL207)
PMID: 8789057Publication date:1995 Type: RT-PCR
Temp: (94C 30sec>56C 120sec>72C 120sec)*30
Primer Name: R1 Type of Usage: Tm: 72 C at salt 1000.0mM
Primer Name: F1 Type of Usage: Tm: Max Tm 74.1 C Min Tm 72 C at salt 1000.0mM

Common Data ID: SW0034 Virus: bovine viral diarrhea virus (isolate NADL), BVDV; Hog cholera virus (strain Alfort), CSFV
PMID: 9054133Publication date:02/96 Type: RT-PCR
Temp: 94C 3min>(94C 1min>65C 1min>72C 1min)*35
Primer Name: P1 Type of Usage: PCRTm: 83.7 C at salt 1000.0mM
Primer Name: P2 Type of Usage: PCRTm: 85.5 C at salt 1000.0mM
Primer Name: P3 Type of Usage: PCRTm: 85.4 C at salt 1000.0mM

Common Data ID: SW0040 Virus: pestivirus type 1, BVDV
PMID: 9403511Publication date:07/97 Type: RT-PCR
Temp: (94C 30sec>55C 100sec>72C 100sec)*30
Primer Name: F Type of Usage: PCRTm: Max Tm 74.1 C Min Tm 72 C at salt 1000.0mM
Primer Name: R Type of Usage: PCRTm: 72 C at salt 1000.0mM

Common Data ID: SW0051 Virus: pestivirus type 1, BVDV
PMID: 9713819Publication date:07/98 Type: RT-PCR
Temp: 94C 2min>(95C 1min>55C 1min>72C 1min)*50>72C 7min
Primer Name: SW00510 Type of Usage: PCRTm: 67.9 C at salt 1000.0mM
Primer Name: SW00511 Type of Usage: PCRTm: 70 C at salt 1000.0mM

Common Data ID: ta0041 Virus: Bovine viral diarrhea virus, BVDV
PMID: 12435688Publication date:12/02 Type: Q-RT-PCR
Temp:
Primer Name: ta00410 Type of Usage: Forward PrimerTm: 73.2 C at salt 1000.0mM
Primer Name: ta00411 Type of Usage: Hybridization ProbeTm: 80.5 C at salt 1000.0mM
Primer Name: ta00412 Type of Usage: Reverse PrimerTm: 72 C at salt 1000.0mM

Common Data ID: YS0012 Virus: Pestivirus type 1
PMID: 14624234Publication date:11/17/2003 Type: Hybridization
Temp:
Primer Name: 10001701 Type of Usage: Tm: 92.26C at salt 1000.0 mM
Primer Name: 10001702 Type of Usage: Tm: 92.26C at salt 1000.0 mM
Primer Name: 10001703 Type of Usage: Tm: 91.09C at salt 1000.0 mM
Primer Name: 10001704 Type of Usage: Tm: 91.09C at salt 1000.0 mM
Primer Name: 10001705 Type of Usage: Tm: 97.53C at salt 1000.0 mM
Primer Name: 10001706 Type of Usage: Tm: 97.53C at salt 1000.0 mM
Primer Name: 10001707 Type of Usage: Tm: 88.74C at salt 1000.0 mM
Primer Name: 10001708 Type of Usage: Tm: 88.74C at salt 1000.0 mM
Primer Name: 10001709 Type of Usage: Tm: 91.09C at salt 1000.0 mM
Primer Name: 10001710 Type of Usage: Tm: 91.09C at salt 1000.0 mM
Primer Name: 10001711 Type of Usage: Tm: 85.81C at salt 1000.0 mM
Primer Name: 10001712 Type of Usage: Tm: 85.81C at salt 1000.0 mM
Primer Name: 10001713 Type of Usage: Tm: 96.94C at salt 1000.0 mM
Primer Name: 10001714 Type of Usage: Tm: 96.94C at salt 1000.0 mM
Primer Name: 10001715 Type of Usage: Tm: 92.26C at salt 1000.0 mM
Primer Name: 10001716 Type of Usage: Tm: 92.26C at salt 1000.0 mM
Primer Name: 10001717 Type of Usage: Tm: 95.77C at salt 1000.0 mM
Primer Name: 10001718 Type of Usage: Tm: 95.77C at salt 1000.0 mM
Primer Name: 10001719 Type of Usage: Tm: 93.43C at salt 1000.0 mM
Primer Name: 10001720 Type of Usage: Tm: 93.43C at salt 1000.0 mM
Primer Name: 10005413 Type of Usage: Tm: 85.23C at salt 1000.0 mM
Primer Name: 10005414 Type of Usage: Tm: 85.23C at salt 1000.0 mM
Primer Name: 10005415 Type of Usage: Tm: 92.26C at salt 1000.0 mM
Primer Name: 10005416 Type of Usage: Tm: 92.26C at salt 1000.0 mM
Primer Name: 10005417 Type of Usage: Tm: 89.91C at salt 1000.0 mM
Primer Name: 10005418 Type of Usage: Tm: 89.91C at salt 1000.0 mM
Primer Name: 10005419 Type of Usage: Tm: 98.7C at salt 1000.0 mM
Primer Name: 10005420 Type of Usage: Tm: 98.7C at salt 1000.0 mM
Primer Name: 10005421 Type of Usage: Tm: 94.01C at salt 1000.0 mM
Primer Name: 10005422 Type of Usage: Tm: 94.01C at salt 1000.0 mM
Primer Name: 10007693 Type of Usage: Tm: 86.99C at salt 1000.0 mM
Primer Name: 10007694 Type of Usage: Tm: 86.99C at salt 1000.0 mM
Primer Name: 10007695 Type of Usage: Tm: 95.19C at salt 1000.0 mM
Primer Name: 10007696 Type of Usage: Tm: 95.19C at salt 1000.0 mM
Primer Name: 10007697 Type of Usage: Tm: 91.09C at salt 1000.0 mM
Primer Name: 10007698 Type of Usage: Tm: 91.09C at salt 1000.0 mM
Primer Name: 10007699 Type of Usage: Tm: 89.33C at salt 1000.0 mM
Primer Name: 10007700 Type of Usage: Tm: 89.33C at salt 1000.0 mM
Primer Name: 10007701 Type of Usage: Tm: 91.09C at salt 1000.0 mM
Primer Name: 10007702 Type of Usage: Tm: 91.09C at salt 1000.0 mM
Primer Name: 10007703 Type of Usage: Tm: 94.6C at salt 1000.0 mM
Primer Name: 10007704 Type of Usage: Tm: 94.6C at salt 1000.0 mM
Primer Name: 10007705 Type of Usage: Tm: 96.94C at salt 1000.0 mM
Primer Name: 10007706 Type of Usage: Tm: 96.94C at salt 1000.0 mM
Primer Name: 10007707 Type of Usage: Tm: 89.33C at salt 1000.0 mM
Primer Name: 10007708 Type of Usage: Tm: 89.33C at salt 1000.0 mM
Primer Name: 10007709 Type of Usage: Tm: 89.91C at salt 1000.0 mM
Primer Name: 10007710 Type of Usage: Tm: 89.91C at salt 1000.0 mM
Primer Name: 10007711 Type of Usage: Tm: 89.91C at salt 1000.0 mM
Primer Name: 10007712 Type of Usage: Tm: 89.91C at salt 1000.0 mM

Total 256 Oligos
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