A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Reverse(BNIinAs) primer
Oligo ID:UM00191
Oligo type: PCR Denegeracy: 1
Target: 18309-18288 Tm: 72.9 C at salt 1000.0mMLength: 22
Sequence: CTGTAGAAAATCCTAGCTGGAG
(7 other experimental conditions)
Note:

Experimental condition used for the oligonucleotide

Common Data ID: UM0019 Publication date: 8/2003
PMID: 12690091 GI: 30271926 Taxonomy ID:227859
Virus Name: SARS Coronavirus Product size (PCR): 108 bp
MgCl2: 2.5 mM dNTPs: 0.2 mM Type: Nested PCR
Temp (PCR cycle):95C 3min>(95C 10sec>60C 10sec[-1C/cyc]>72C 20sec)*10>(95C 10sec>56C 10sec>72C 20sec)*20
Buffer: InVitrogen
Polymerase: 0.025 units/microL platinum Taq pol (InVitrogen)Cycler:
Note:



Oligonucleotide information
Reverse(BNIinAs) primer
Oligo ID:UM00191
Oligo type: PCR Denegeracy: 1
Target: 18309-18288 Tm: 72.9 C at salt 1000.0mMLength: 22
Sequence: CTGTAGAAAATCCTAGCTGGAG
(7 other experimental conditions)
Note:

Experimental condition used for the oligonucleotide

Common Data ID: SA0002 Publication date: 12/03
PMID: 15070991 GI: 30271926 Taxonomy ID:227859
Virus Name: SARS coronavirus Product size (PCR): 109 bp
MgCl2: mM dNTPs: mM Type: Nested RT-PCR
Temp (PCR cycle):
Buffer: PCR amplification master mix
Polymerase: Superscript II/Platinum TaqCycler:
Note:



Oligonucleotide information
Reverse(BNIinAs) primer
Oligo ID:UM00191
Oligo type: PCR Denegeracy: 1
Target: 18309-18288 Tm: 72.9 C at salt 1000.0mMLength: 22
Sequence: CTGTAGAAAATCCTAGCTGGAG
(7 other experimental conditions)
Note:

Experimental condition used for the oligonucleotide

Common Data ID: UM0019 Publication date: 8/2003
PMID: 12690091 GI: 30271926 Taxonomy ID:227859
Virus Name: SARS Coronavirus Product size (PCR): 108 bp
MgCl2: 2.5 mM dNTPs: 0.2 mM Type: Nested PCR
Temp (PCR cycle):95C 3min>(95C 10sec>60C 10sec[-1C/cyc]>72C 20sec)*10>(95C 10sec>56C 10sec>72C 20sec)*20
Buffer: InVitrogen
Polymerase: 0.025 units/microL platinum Taq pol (InVitrogen)Cycler:
Note:



Oligonucleotide information
Reverse(BNIinAs) primer
Oligo ID:UM00191
Oligo type: PCR Denegeracy: 1
Target: 18309-18288 Tm: 72.9 C at salt 1000.0mMLength: 22
Sequence: CTGTAGAAAATCCTAGCTGGAG
(7 other experimental conditions)
Note:

Experimental condition used for the oligonucleotide

Common Data ID: SA0002 Publication date: 12/03
PMID: 15070991 GI: 30271926 Taxonomy ID:227859
Virus Name: SARS coronavirus Product size (PCR): 109 bp
MgCl2: mM dNTPs: mM Type: Nested RT-PCR
Temp (PCR cycle):
Buffer: PCR amplification master mix
Polymerase: Superscript II/Platinum TaqCycler:
Note:

Total 4 Oligos
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