A Simple Solution for Oligonucleotide Design

Click here for a list of viruses in the database

Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

°C± °C
Oligo length:
bp± bp


Common Data ID:

Taxonomy ID:

GI Number:

Oligonucleotide information
Reverse(N2.4) primer
Oligo ID:SW00813
Oligo type: PCR Denegeracy: 1
Target: 857-838 Tm: 67.9 C at salt 1000.0mMLength: 20
(1 other experimental conditions)
Note: Used with N2.3 for secondary PCR.

Experimental condition used for the oligonucleotide

Common Data ID: SW0081 Publication date: 06/99
PMID: 10325337 GI: 450251 Taxonomy ID:11246
Virus Name: Bovine respiratory syncytial virus, BRSV Product size (PCR):
MgCl2: 1.0 mM dNTPs: 0.2 mM Type: RT-Nested PCR
Temp (PCR cycle):94C 12min>(94C 60sec>58C 60sec>72C 90sec)*35>72C 10min
Buffer: 10mM Tris-HCl (pH 8.3), 50mM KCl
Polymerase: 0.025U/microL AmpliTaq Gold poly (Perkin Elmer Cetus)Cycler: GeneAmp PCR System 480 (Perkin Elmer Cetus)
Note: Used with N2.3 for secondary PCR.

Total 1 Oligos
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