A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Forward(N2.1) primer
Oligo ID:SW00810
Oligo type: PCR Denegeracy: 1
Target: 1-19 Tm: 71.5 C at salt 1000.0mMLength: 19
Sequence: ATGGCTCTCAGCAAGGTCA
(1 other experimental conditions)
Note: Used with N2.2 for primary PCR.

Experimental condition used for the oligonucleotide

Common Data ID: SW0081 Publication date: 06/99
PMID: 10325337 GI: 450251 Taxonomy ID:11246
Virus Name: Bovine respiratory syncytial virus, BRSV Product size (PCR):
bp
MgCl2: 1.0 mM dNTPs: 0.2 mM Type: RT-Nested PCR
Temp (PCR cycle):94C 12min>(94C 60sec>58C 60sec>72C 90sec)*35>72C 10min
Buffer: 10mM Tris-HCl (pH 8.3), 50mM KCl
Polymerase: 0.025U/microL AmpliTaq Gold poly (Perkin Elmer Cetus)Cycler: GeneAmp PCR System 480 (Perkin Elmer Cetus)
Note: Used with N2.2 for primary PCR.

Total 1 Oligos
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