A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Forward(D1) primer
Oligo ID:SW00220
Oligo type: PCR Denegeracy: 1
Target: 1053-1070 Tm: 64 CLength: 18
Sequence: TACCTGCGCAGCGGGCGC
(0 other experimental conditions)
Note: Used with R1 for primary PCR.

Experimental condition used for the oligonucleotide

Common Data ID: SW0022 Publication date: 04/96
PMID: 8785714 GI: 330756 Taxonomy ID:10320
Virus Name: Bovine herpesvirus 1, BHV-1 Product size (PCR):
bp
MgCl2: 2.5 mM dNTPs: 0.2 mM Type: Nested PCR
Temp (PCR cycle):94C 5min>(94C 60sec>55C 90sec>72C 120sec)*30>72C 5min
Buffer: 10mM Tris-HCl (pH8.3), 50mM KCl, 2% formamide
Polymerase: 0.025U/microL Taq DNA poly (Gibco/BRL)Cycler: Robocycler thermocycler
Note: Used with R1 for primary PCR.

Total 1 Oligos
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