A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Reverse(SW00163) primer
Oligo ID:SW00163
Oligo type: PCR Denegeracy: 1
Target: 1007-990 Tm: 58 CLength: 18
Sequence: AGCCCTCGATCTGCTGGA
(2 other experimental conditions)
Note: Reverse primer for second nested PCR

Experimental condition used for the oligonucleotide

Common Data ID: SW0016 Publication date: 05/92
PMID: 1314454 GI: 330745 Taxonomy ID:10323
Virus Name: Bovine herpesvirus type 1.1 (strain Cooper) Product size (PCR): 353 bp
MgCl2: 2.5 mM dNTPs: 0.2 mM Type: PCR
Temp (PCR cycle):(94C 2min>67C 1min>74C 1min)*40>74C 5min
Buffer: 10mM Tris-HCl (pH 8.3), 50mM KCl, 4.0% dimethyl sulfoxide
Polymerase: 0.025U/microL AmpliTaq poly (Perkin-Elmer Cetus)Cycler: Model 50 Tempcycler (Coy Laboratory Prod. Inc.)
Note: Reverse primer for second nested PCR

Total 1 Oligos
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