A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Forward(Sensor probe MGB pox 14kd) primer
Oligo ID:SG013806
Oligo type: PCR Probe Denegeracy: 1
Target: 131862-131882 Tm: 66.6C at salt 1000.0 mMLength: 21
Sequence: CCAACCTAAATAGIACTTCAT
(0 other experimental conditions)
Note: This probe has a 6-carboxyfluorescein group on the 5'end and a Quencher on the 3'end. One discrepancy was found in Blast search the nucleotide at 131872 was a G instead of a T as reported by the article.

Experimental condition used for the oligonucleotide

Common Data ID: SG0138 Publication date: 05/04
PMID: 15131152 GI: 5830555 Taxonomy ID:10255
Virus Name: Variola virus Product size (PCR): bp
MgCl2: mM dNTPs: mM Type: Real-time PCR
Temp (PCR cycle):95C 10sec>(95C 5sec>55C 15sec>72C 15sec)*45
Buffer:
Polymerase: Cycler: LightCycler (Roche Diagnostics)
Note: This probe has a 6-carboxyfluorescein group on the 5'end and a Quencher on the 3'end. One discrepancy was found in Blast search the nucleotide at 131872 was a G instead of a T as reported by the article.

Total 1 Oligos
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