A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Forward(VARJ7R3P) primer
Oligo ID:SG011103
Oligo type: PCR Probe Denegeracy: 1
Target: 151652-151677 Tm: 75.8C at salt 1000.0 mMLength: 26
Sequence: CAAGACGTCGGGACCAATTACTAATA
(1 other experimental conditions)
Note: The probe contains a 6-carboxyfluorescein at the 5' end and a 6-carboxytetramethylrhodamine and a phosphate at the 3' end.

Experimental condition used for the oligonucleotide

Common Data ID: SG0111 Publication date: 08/03
PMID: 12904397 GI: 623595 Taxonomy ID:12870
Virus Name: Variola major virus Product size (PCR): bp
MgCl2: 5 mM dNTPs: 0.2 mM Type: Real-time PCR
Temp (PCR cycle):95C 120sec>(95C 10sec>60C 45sec)*45
Buffer: 50mM Tris (pH 8.3), 25microG bovine serum albumin per ml
Polymerase: 1.35U Platinum Taq poly (Invitrogen)Cycler: Smart Cycler
Note: The probe contains a 6-carboxyfluorescein at the 5' end and a 6-carboxytetramethylrhodamine and a phosphate at the 3' end.



Oligonucleotide information
Forward(VARJ7R3P) primer
Oligo ID:SG011103
Oligo type: PCR Probe Denegeracy: 1
Target: 151652-151677 Tm: 75.8C at salt 1000.0 mMLength: 26
Sequence: CAAGACGTCGGGACCAATTACTAATA
(1 other experimental conditions)
Note: The probe contains a 6-carboxyfluorescein at the 5' end and a 6-carboxytetramethylrhodamine and a phosphate at the 3' end.

Experimental condition used for the oligonucleotide

Common Data ID: SG0112 Publication date: 08/03
PMID: 12904397 GI: 623595 Taxonomy ID:12870
Virus Name: Variola major virus Product size (PCR): bp
MgCl2: 7 mM dNTPs: 0.2 mM Type: Real-time PCR
Temp (PCR cycle):95C 120sec>(95C 1sec>60C 20sec)*45
Buffer: 50mM Tris (pH 8.3), 25microG bovine serum albumin per mL
Polymerase: 0.8U Platinum TaqDNA (Invitrogen)Cycler: LightCycler
Note: The probe contains a 6-carboxyfluorescein at the 5' end and a 6-carboxytetramethylrhodamine and a phosphate at the 3' end.



Oligonucleotide information
Forward(VARJ7R3P) primer
Oligo ID:sg011103
Oligo type: PCR Probe Denegeracy: 1
Target: 151652-151677 Tm: 75.8C at salt 1000.0 mMLength: 26
Sequence: CAAGACGTCGGGACCAATTACTAATA
(1 other experimental conditions)
Note: The probe contains a 6-carboxyfluorescein at the 5' end and a 6-carboxytetramethylrhodamine and a phosphate at the 3' end.

Experimental condition used for the oligonucleotide

Common Data ID: SG0111 Publication date: 08/03
PMID: 12904397 GI: 623595 Taxonomy ID:12870
Virus Name: Variola major virus Product size (PCR): bp
MgCl2: 5 mM dNTPs: 0.2 mM Type: Real-time PCR
Temp (PCR cycle):95C 120sec>(95C 10sec>60C 45sec)*45
Buffer: 50mM Tris (pH 8.3), 25microG bovine serum albumin per ml
Polymerase: 1.35U Platinum Taq poly (Invitrogen)Cycler: Smart Cycler
Note: The probe contains a 6-carboxyfluorescein at the 5' end and a 6-carboxytetramethylrhodamine and a phosphate at the 3' end.



Oligonucleotide information
Forward(VARJ7R3P) primer
Oligo ID:sg011103
Oligo type: PCR Probe Denegeracy: 1
Target: 151652-151677 Tm: 75.8C at salt 1000.0 mMLength: 26
Sequence: CAAGACGTCGGGACCAATTACTAATA
(1 other experimental conditions)
Note: The probe contains a 6-carboxyfluorescein at the 5' end and a 6-carboxytetramethylrhodamine and a phosphate at the 3' end.

Experimental condition used for the oligonucleotide

Common Data ID: SG0112 Publication date: 08/03
PMID: 12904397 GI: 623595 Taxonomy ID:12870
Virus Name: Variola major virus Product size (PCR): bp
MgCl2: 7 mM dNTPs: 0.2 mM Type: Real-time PCR
Temp (PCR cycle):95C 120sec>(95C 1sec>60C 20sec)*45
Buffer: 50mM Tris (pH 8.3), 25microG bovine serum albumin per mL
Polymerase: 0.8U Platinum TaqDNA (Invitrogen)Cycler: LightCycler
Note: The probe contains a 6-carboxyfluorescein at the 5' end and a 6-carboxytetramethylrhodamine and a phosphate at the 3' end.

Total 4 Oligos
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