A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Forward(SG004403) primer
Oligo ID:SG004403
Oligo type: PCR Probe Denegeracy: 1
Target: 17745-17760 Tm: 50CLength: 16
Sequence: TCTGCGTAGGCAATCC
(2 other experimental conditions)
Note: The probe has a 6-carboxyfluorescein on the 5' end and a nonfluorescent quencher on the 3' end.

Experimental condition used for the oligonucleotide

Common Data ID: SG0044 Publication date: 2004
PMID: 14709637 GI: 30023963 Taxonomy ID:228404
Virus Name: SARS coronavirus HKU-39849 Product size (PCR): 43 bp
MgCl2: 5.5 mM dNTPs: 0.2 mM Type: Real-time RT-PCR
Temp (PCR cycle):50C 120sec>95C 600sec>(95C 15sec>60C 60sec)*45
Buffer: 1X Taqman buffer A
Polymerase: 0.625U AmpliTaq Gold polymerase (Applied Biosystems)Cycler: 7000 Sequence Detection System (Applied Biosystems)
Note: The probe has a 6-carboxyfluorescein on the 5' end and a nonfluorescent quencher on the 3' end.

Total 1 Oligos
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