A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Reverse(BARN) primer
Oligo ID:SG003220
Oligo type: Reverse Primer Denegeracy: 48
Target: 19769-19747 Tm: max Tm 69.7C min Tm 66.1C at salt 1000.0mMLength: 23
Sequence: CC(A/T)GAATA(A/C/T)(A/G)IAAA(A/G)TT(G/T)GGATC
(0 other experimental conditions)
Note: Nested primer.

Experimental condition used for the oligonucleotide

Common Data ID: SG0032 Publication date: 03/04
PMID: 15006765 GI: 34420972 Taxonomy ID:70333
Virus Name: Bovine adenovirus 4 Product size (PCR): 430 bp
MgCl2: 1.5 mM dNTPs: 0.025 mM Type: Nested PCR
Temp (PCR cycle):94C 240sec>(92C 60sec>55C 60sec>72C 75sec)*29>72C 420sec
Buffer: 1X PCR buffer [160mM (NH4)2SO4-670mM Tris-HCl(pH 8.8)
Polymerase: 2U Taq poly (Bioron GmbH)Cycler: Gene Amp PCR 2400 (Applied Biosystems)
Note: Nested primer.

Total 1 Oligos
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