A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Forward(PN00423) primer
Oligo ID:pn00423
Oligo type: Denegeracy: 1
Target: 188-205 Tm: 56 CLength: 18
Sequence: GTAGTCGTCAGTGGTTCG
(3 other experimental conditions)
Note: Used with PN00424, [GI:9626649], in primary PCR.

Experimental condition used for the oligonucleotide

Common Data ID: BH0057 Publication date: 12/98
PMID: 9850544 GI: 902376 Taxonomy ID:54315
Virus Name: Bovine viral diarrhea virus-2, BVDV2; Bovine viral diarrhea virus (isolate NADL), BVDV-NADL Product size (PCR):
bp
MgCl2: 1.5 mM dNTPs: 0.2 mM Type: RT-Nested PCR
Temp (PCR cycle):94C 2min>(94C 30sec>50C 45sec>72C 60sec)*30>72C 7min
Buffer: 20mM Tris-HCl (pH 8.4), 50mM KCl
Polymerase: 0.05U/microL Taq polyCycler:
Note: Used with PN00424, [GI:9626649], in primary PCR.

Total 1 Oligos
Copyright © 2001-2005 VirOligo Database Project. All rights reserved Feedback is welcome. Contact webmaster Ulrich Melcher at ulrich.melcher@okstate.edu.