A Simple Solution for Oligonucleotide Design

Click here for a list of viruses in the database

Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

°C± °C
Oligo length:
bp± bp


Common Data ID:

Taxonomy ID:

GI Number:

Oligonucleotide information
Forward(BTKF2) primer
Oligo ID:pn00268
Oligo type: Denegeracy: 1
Target: 62810-62828 Tm: 73.7 C at salt 1000.0mMLength: 19
(0 other experimental conditions)
Note: Mismatch at position 62825.

Experimental condition used for the oligonucleotide

Common Data ID: SK0062 Publication date: 2000
PMID: 10971126 GI: 9629818 Taxonomy ID:10320
Virus Name: Bovine herpesvirus 1, BHV-1 Product size (PCR): 179 bp
MgCl2: 1.5 mM dNTPs: 0.2 mM Type: PCR
Temp (PCR cycle):97C 3min>61C 3min>72C 3min>(97C 1min>61C 1min>72C 1min)*20>(97C 30sec>61C 30sec>72C 30sec)*40
Buffer: 1x PCR buffer (Gibco BRL)
Polymerase: 0.025U/microL Taq DNA poly (Gibco BRL)Cycler: GeneAmp PCR System 2400 (Perkin-Elmer)
Note: Mismatch at position 62825.

Total 1 Oligos
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