A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Forward(5'UTR 1) primer
Oligo ID:PN00035
Oligo type: Denegeracy: 1
Target: 100-117 Tm: 58 CLength: 18
Sequence: GGCTAGCCATGCCCTTAG
(4 other experimental conditions)
Note: Used with 5'UTR 2.

Experimental condition used for the oligonucleotide

Common Data ID: SK0002 Publication date: 04/95
PMID: 7667908 GI: 9626649 Taxonomy ID:31656
Virus Name: Bovine viral diarrhea virus (strain SD-1), BVDV strain SD-1 Product size (PCR):
bp
MgCl2: 2.2 mM dNTPs: 0.2 mM Type: RT-PCR
Temp (PCR cycle):94C 4min>(94C 1min>55C 1.5min>72C 3min)*30>72C 7min
Buffer: 18mM Tris-HCl (pH 8.3), 55mM KCl, 2mM dithiothreitol
Polymerase: 0.025U/microL AmpliTaq poly (Perkin-Elmer Cetus Corp.)Cycler: DNA thermal cycler (Coy Laboratory Products Inc.; Ann Arbor, MI)
Note: Used with 5'UTR 2.

Total 1 Oligos
Copyright © 2001-2005 VirOligo Database Project. All rights reserved Feedback is welcome. Contact webmaster Ulrich Melcher at ulrich.melcher@okstate.edu.