A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Forward(BVDu2) primer
Oligo ID:PM00504
Oligo type: Denegeracy: 8
Target: 227-244 Tm: Max Tm 58 C Min Tm 52 CLength: 18
Sequence: GA(G/T)ATGC(C/T)A(C/T)GTGGACGA
(0 other experimental conditions)
Note:

Experimental condition used for the oligonucleotide

Common Data ID: MP0865 Publication date: 12/02
PMID: 12421586 GI: 7960753 Taxonomy ID:11100
Virus Name: Bovine viral diarrhea virus (isolate NADL), BVDV Product size (PCR):
bp
MgCl2: 2.5 mM dNTPs: 0.2 mM Type: RT-Semi-nested PCR
Temp (PCR cycle):95C 3min>(95C 30sec>55C 30sec>72C 60sec)*25>72C 3min
Buffer: 1x reaction buffer (Promega), 0.02% BSA
Polymerase: 0.02U/microL Taq DNA poly (Promega)Cycler: PTC 200 thermal cycler (MJ Research)
Note:

Total 1 Oligos
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