A Simple Solution for Oligonucleotide Design

Click here for a list of viruses in the database

Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

°C± °C
Oligo length:
bp± bp


Common Data ID:

Taxonomy ID:

GI Number:

Oligonucleotide information
Forward(PJ00327) primer
Oligo ID:pj00327
Oligo type: Denegeracy: 1
Target: 3194-3213 Tm: 70 C at salt 1000.0mMLength: 20
(0 other experimental conditions)
Note: Mismatch at position 3202.

Experimental condition used for the oligonucleotide

Common Data ID: BM0489 Publication date: 06/94
PMID: 8079512 GI: 10445391 Taxonomy ID:12120
Virus Name: Foot-and-mouth disease virus (strain C1-Santa Pau) Product size (PCR):
MgCl2: 1.6 mM dNTPs: 0.04 mM Type: RT-PCR
Temp (PCR cycle):(94C 1min>55C 1min>72C 2min)*30>72C 8min
Buffer: 10mM Tris-HCl (pH 8.3), 4.0mM KCl
Polymerase: 0.025U/microL Taq poly (Promega)Cycler: DNA thermal cycler (Perkin Elmer)
Note: Mismatch at position 3202.

Total 1 Oligos
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