A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Forward(F1) primer
Oligo ID:pj00189
Oligo type: Denegeracy: 4
Target: 102-121 Tm: Max Tm 74.1 C Min Tm 72 C at salt 1000.0mMLength: 20
Sequence: ATGCCC(A/T)(C/T)AGTAGGACTAGC
(4 other experimental conditions)
Note:

Experimental condition used for the oligonucleotide

Common Data ID: BM0559 Publication date: 2000
PMID: 11145272 GI: 15282441 Taxonomy ID:155905
Virus Name: pestivirus Giraffe-1 Product size (PCR):
bp
MgCl2: 1.5 mM dNTPs: 0.2 mM Type: RT-PCR
Temp (PCR cycle):(94C 30sec>55C 120sec>72C 120sec)*30
Buffer: 10.6mM Tris-HCl, 98mM KCl, 500microg/mL BSA, 0.1% sodium chlorate, 0.1% Triton X-100, 6microM EDTA, 3% glycerol
Polymerase: 0.025U/microL Tth DNA polyCycler:
Note:

Total 1 Oligos
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