A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Forward(TS2) primer
Oligo ID:PE00009
Oligo type: Denegeracy: 1
Target: 1802-1821 Tm: 70 C at salt 1000.0mMLength: 20
Sequence: TGGTTAGGGAAGCAATTAGG
(1 other experimental conditions)
Note: Mismatches at position 1810, 1816, 1817, 1819.

Experimental condition used for the oligonucleotide

Common Data ID: JP0041 Publication date: 01/02
PMID: 11808702 GI: 902376 Taxonomy ID:136447
Virus Name: bovine viral diarrhea virus type 2, BVDV 2 Product size (PCR): N/A bp
MgCl2: 1.5 mM dNTPs: 0.2 mM Type: RT-Semi-Nested PCR 2
Temp (PCR cycle):94C 2min>(94C 1min>50C 1min>72C 1min)*30>72C 10min
Buffer: 1x PCR buffer
Polymerase: 0.05U/microL Taq polyCycler: PTC-100 thermal cycler(MJ research, Waltham MA, USA)
Note: Mismatches at position 1810, 1816, 1817, 1819.

Total 1 Oligos
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