A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Forward(ATI-up-1) primer
Oligo ID:pd00518
Oligo type: Denegeracy: 1
Target: 1715-1730 Tm: 44 CLength: 16
Sequence: AATACAAGGAGGATCT
(2 other experimental conditions)
Note:

Experimental condition used for the oligonucleotide

Common Data ID: JX0068 Publication date: 03/97
PMID: 9079767 GI: 1916855 Taxonomy ID:10255
Virus Name: Variola virus; Monkeypox virus; Cowpox virus; Vaccinia virus (strain WR); Ectromelia virus; camelpox virus; volepox virus; Raccoonpox virus; skunkpox virus Product size (PCR):
bp
MgCl2: 2.5 mM dNTPs: 0.2 mM Type: ATI-PCR
Temp (PCR cycle):(94C 1min>40C 1min>72C 2.5min)*25>72C 10min
Buffer: 50mM KCl, 10mM Tris-HCl pH 8.3
Polymerase: 0.05U/microL Taq poly (Boehringer-GmbH, Germany)Cycler: GeneAmp PCR System 9600 (Perkin-Elmer Cetus, Norwalk, CT)
Note:



Oligonucleotide information
Forward(ATI-up-1) primer
Oligo ID:pd00518
Oligo type: Denegeracy: 1
Target: 1715-1730 Tm: 44 CLength: 16
Sequence: AATACAAGGAGGATCT
(2 other experimental conditions)
Note:

Experimental condition used for the oligonucleotide

Common Data ID: JX0068 Publication date: 03/97
PMID: 9079767 GI: 1916855 Taxonomy ID:10255
Virus Name: Variola virus; Monkeypox virus; Cowpox virus; Vaccinia virus (strain WR); Ectromelia virus; camelpox virus; volepox virus; Raccoonpox virus; skunkpox virus Product size (PCR):
bp
MgCl2: 2.5 mM dNTPs: 0.2 mM Type: ATI-PCR
Temp (PCR cycle):(94C 1min>40C 1min>72C 2.5min)*25>72C 10min
Buffer: 50mM KCl, 10mM Tris-HCl pH 8.3
Polymerase: 0.05U/microL Taq poly (Boehringer-GmbH, Germany)Cycler: GeneAmp PCR System 9600 (Perkin-Elmer Cetus, Norwalk, CT)
Note:

Total 2 Oligos
Copyright © 2001-2005 VirOligo Database Project. All rights reserved Feedback is welcome. Contact webmaster Ulrich Melcher at ulrich.melcher@okstate.edu.