A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Reverse(VL33) primer
Oligo ID:pd00490
Oligo type: Denegeracy: 1
Target: 6209-6191 Tm: 62.9 C at salt 1000.0mMLength: 19
Sequence: ACCATTACAAACATTATCC
(0 other experimental conditions)
Note: There was a mismatch at position 6198.
Primer anneals as reverse primer at positions 6209-6191 and as a forward primer at positions 185529-185547.

Experimental condition used for the oligonucleotide

Common Data ID: JX0086 Publication date: 01/01
PMID: 11136755 GI: 9790357 Taxonomy ID:10243
Virus Name: Cowpox virus; Ectromelia virus; Taterapox virus; Vaccinia virus (strain Lister); Buffalopox virus; Rabbitpox virus (strain Utrecht); Variola major virus; variola minor virus; Monkeypox virus; Camelpox virus Product size (PCR):
bp
MgCl2: N/A mM dNTPs: N/A mM Type: Universal PCR
Temp (PCR cycle):(94C 1min>55C 1min>72C 1min)*25>72C 5min
Buffer: N/A
Polymerase: N/ACycler: model 9600 thermalcycler (Perkin-Elmer Cetus)
Note: There was a mismatch at position 6198.
Primer anneals as reverse primer at positions 6209-6191 and as a forward primer at positions 185529-185547.

Total 1 Oligos
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