A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Forward(Oligo 5) primer
Oligo ID:pd00420
Oligo type: Denegeracy: 1
Target: 6700-6719 Tm: 76.1 C at salt 1000.0mMLength: 20
Sequence: TTCGAAACGGCACGGTCGGA
(0 other experimental conditions)
Note: Sequence given in article lacks a "G" between positions 6704 and 6705 when compared with BLAST.

Experimental condition used for the oligonucleotide

Common Data ID: BM0483 Publication date: 03/92
PMID: 1313822 GI: 61076 Taxonomy ID:73482
Virus Name: Foot-and-mouth disease virus (strain O1); Foot-and-mouth disease virus Asia 1 Product size (PCR):
bp
MgCl2: 1.5 mM dNTPs:
mM
Type: RT-PCR
Temp (PCR cycle):(93C 30sec>55C 30sec>72C 60sec)*35>72C 9min
Buffer: 10mM Tris-HCl (pH 8.3) 50mM KCl
Polymerase: 0.08U/microL Taq DNA poly (IBI)Cycler: programmable thermal controller (MJ Research Inc.)
Note: Sequence given in article lacks a "G" between positions 6704 and 6705 when compared with BLAST.

Total 1 Oligos
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