A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Reverse(3D2) primer
Oligo ID:PD00197
Oligo type: Denegeracy: 1
Target: 6524-6506 Tm: 75.8 C at salt 1000.0mMLength: 19
Sequence: GCGGAACAGCGCTTTGTCC
(2 other experimental conditions)
Note:

Experimental condition used for the oligonucleotide

Common Data ID: BM0306 Publication date: 06/98
PMID: 9694330 GI: 61076 Taxonomy ID:12120
Virus Name: Foot-and-mouth disease virus (strain C1-Santa Pau), FMDV C-S8 Sp70; Foot-and-mouth disease virus (strain O1), FMDV O1 Kb; Foot-and-mouth disease virus (strain A5), FMDV A5 Ww Product size (PCR):
bp
MgCl2: 1.5 mM dNTPs: 100 mM Type: RT-PCR
Temp (PCR cycle):93C 10min>(93C 1min>37C 1min>72C 2min)*30
Buffer: 10mM Tris-HCl (pH 8.3), 50mM KCl, 0.01% gelatine, 10mM DTT, 0.4U/microL ribonuclease inhibitor, 0.1U/microL AMV RTase
Polymerase: 0.025U/microL Taq Gold poly (Perkin-Elmer, Cetus)Cycler:
Note:



Oligonucleotide information
Reverse(3D2) primer
Oligo ID:PD00197
Oligo type: Denegeracy: 1
Target: 6524-6506 Tm: 75.8 C at salt 1000.0mMLength: 19
Sequence: GCGGAACAGCGCTTTGTCC
(2 other experimental conditions)
Note:

Experimental condition used for the oligonucleotide

Common Data ID: BM0309 Publication date: 06/98
PMID: 9694330 GI: 402536 Taxonomy ID:12075
Virus Name: Swine vesicular disease virus (isolate Spain1/93); Foot-and-mouth disease virus (strain C1-Santa Pau); Vesicular stomatitis New Jersey virus, VSV NJ; Vesicular stomatitis Indiana virus, VSV Ind Product size (PCR):
bp
MgCl2: 1.5 mM dNTPs: 100 mM Type: Multiplex RT-PCR
Temp (PCR cycle):93C 10min>(93C 1min>37C 1min>72C 2min)*30
Buffer: 10mM Tris-HCl (pH 8.3), 50mM KCl, 0.01% gelatine, 10mM DTT, 0.4U/microL ribonuclease inhibitor, 0.1U/microL AMV RTase
Polymerase: 0.025U/microL Taq Gold poly (Perkin-Elmer, Cetus)Cycler:
Note:



Oligonucleotide information
Reverse(3D2) primer
Oligo ID:pd00197
Oligo type: Denegeracy: 1
Target: 6524-6506 Tm: 75.8 C at salt 1000.0mMLength: 19
Sequence: GCGGAACAGCGCTTTGTCC
(2 other experimental conditions)
Note:

Experimental condition used for the oligonucleotide

Common Data ID: BM0306 Publication date: 06/98
PMID: 9694330 GI: 61076 Taxonomy ID:12120
Virus Name: Foot-and-mouth disease virus (strain C1-Santa Pau), FMDV C-S8 Sp70; Foot-and-mouth disease virus (strain O1), FMDV O1 Kb; Foot-and-mouth disease virus (strain A5), FMDV A5 Ww Product size (PCR):
bp
MgCl2: 1.5 mM dNTPs: 100 mM Type: RT-PCR
Temp (PCR cycle):93C 10min>(93C 1min>37C 1min>72C 2min)*30
Buffer: 10mM Tris-HCl (pH 8.3), 50mM KCl, 0.01% gelatine, 10mM DTT, 0.4U/microL ribonuclease inhibitor, 0.1U/microL AMV RTase
Polymerase: 0.025U/microL Taq Gold poly (Perkin-Elmer, Cetus)Cycler:
Note:



Oligonucleotide information
Reverse(3D2) primer
Oligo ID:pd00197
Oligo type: Denegeracy: 1
Target: 6524-6506 Tm: 75.8 C at salt 1000.0mMLength: 19
Sequence: GCGGAACAGCGCTTTGTCC
(2 other experimental conditions)
Note:

Experimental condition used for the oligonucleotide

Common Data ID: BM0309 Publication date: 06/98
PMID: 9694330 GI: 402536 Taxonomy ID:12075
Virus Name: Swine vesicular disease virus (isolate Spain1/93); Foot-and-mouth disease virus (strain C1-Santa Pau); Vesicular stomatitis New Jersey virus, VSV NJ; Vesicular stomatitis Indiana virus, VSV Ind Product size (PCR):
bp
MgCl2: 1.5 mM dNTPs: 100 mM Type: Multiplex RT-PCR
Temp (PCR cycle):93C 10min>(93C 1min>37C 1min>72C 2min)*30
Buffer: 10mM Tris-HCl (pH 8.3), 50mM KCl, 0.01% gelatine, 10mM DTT, 0.4U/microL ribonuclease inhibitor, 0.1U/microL AMV RTase
Polymerase: 0.025U/microL Taq Gold poly (Perkin-Elmer, Cetus)Cycler:
Note:

Total 4 Oligos
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