A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Forward(PD00080) primer
Oligo ID:PD00080
Oligo type: Denegeracy: 2
Target: 10385-10404 Tm: Max Tm 72 C Min Tm 70 C at salt 1000.0mMLength: 20
Sequence: AAGATCCACCCTTATGA(A/G)GC
(1 other experimental conditions)
Note: Mismatches at positions 10390, 10397, and 10400.

Experimental condition used for the oligonucleotide

Common Data ID: MP0299 Publication date: 06/99
PMID: 10325368 GI: 7960753 Taxonomy ID:11100
Virus Name: bovine viral diarrhea virus (isolate NADL), BVDV Product size (PCR): N/A bp
MgCl2: 2 mM dNTPs: 0.2 mM Type: RT-Nested Multiplex PCR 1
Temp (PCR cycle):(94C 20sec>50C 30sec>72C 30sec)*25>72C 15min
Buffer: 20mM Tris-HCl [pH 8.4], 50mM KCl
Polymerase: 0.025U/microL Taq DNA poly (Canadian Life Technologies)Cycler: N/A
Note: Mismatches at positions 10390, 10397, and 10400.

Total 1 Oligos
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