A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Forward(PB00935) primer
Oligo ID:pb00935
Oligo type: Denegeracy: 4
Target: 233-252 Tm: Max Tm 82.3 C Min Tm 78.2 C at salt 1000.0mMLength: 20
Sequence: CCA(C/T)GTGGACGAGGGCA(C/T)GC
(0 other experimental conditions)
Note: 5'labelled with 6-caroxy-fluorescein and 3'labelled with 6-carboxy-tetra-methyl-rhodamine.

Experimental condition used for the oligonucleotide

Common Data ID: BM1351 Publication date: 03/02
PMID: 11939332 GI: 9049956 Taxonomy ID:11099
Virus Name: pestivirus type 1; Bovine viral diarrhea virus genotype 2 Product size (PCR):
bp
MgCl2: 12.5 mM dNTPs: 0.4 mM Type: RT-PCR
Temp (PCR cycle):50C 30min>95C 15min>(94C 1min>55C 1min>72C 2min)*40>72C 7min
Buffer: 1x RT-PCR buffer (One Step RT-PCR kit; Qiagen), 1x TaqMan buffer A (Applied Biosystems), 0.08U/microL RNase inhibitor, 1microL enzyme mix (Qiagen)
Polymerase: HotStart Taq poly (Qiagen)Cycler:
Note: 5'labelled with 6-caroxy-fluorescein and 3'labelled with 6-carboxy-tetra-methyl-rhodamine.

Total 1 Oligos
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