A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Forward(P56) primer
Oligo ID:pb00519
Oligo type: Denegeracy: 1
Target: 3595-3614 Tm: 74.1 C at salt 1000.0mMLength: 20
Sequence: GGCGTTGAGAAACAGCTGTG
(0 other experimental conditions)
Note: Target given in article.
Specific to 1D region

Experimental condition used for the oligonucleotide

Common Data ID: BM0287 Publication date: 1996
PMID: 8634024 GI: 61076 Taxonomy ID:12115
Virus Name: Foot-and-mouth disease virus (strain A24 Cruzeiro); foot-and-mouth disease virus C3, Resende; Foot-and-mouth disease virus (strain O1), BFS 1967; Foot-and-mouth disease virus Asia 1, Ind 9/89; Foot-and-mouth disease virus SAT 2, Knp 5/91/2 Product size (PCR):
bp
MgCl2: 1.5 mM dNTPs: 0.05 mM Type: Multiplex PCR
Temp (PCR cycle):95C 1min>(95C 15sec>60C 60sec)*35>60C 6min
Buffer: 10mM Tris-HCl (pH 8.8), 50mM KCl, 0.1% Triton X-100
Polymerase: 0.025U/microL Taq poly (Promega)Cycler: GeneAmp CR System 9600 (Perkin Elmer)
Note: Target given in article.
Specific to 1D region

Total 1 Oligos
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