A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Forward(BVD 180) primer
Oligo ID:PB00491
Oligo type: Denegeracy: 3
Target: 176-197 Tm: Max Tm 78.5 C Min Tm 76.6 C at salt 1000.0mMLength: 22
Sequence: CCTGAGTACAGGG(A/G/T)AGTCGTCA
(1 other experimental conditions)
Note: Used with HCV 368 to amplify a 213bp product.

Experimental condition used for the oligonucleotide

Common Data ID: MP0258 Publication date: 10/00
PMID: 11131328 GI: 9629506 Taxonomy ID:31656
Virus Name: bovine viral diarrhea virus (strain SD-1), BVDV Product size (PCR): 213 bp
MgCl2: 4 mM dNTPs: 0.4 mM Type: RT- Nested PCR 2
Temp (PCR cycle):(94C 1min>55C 1min>72C 45 sec)*30>72C 10min
Buffer: Same as first PCR
Polymerase: 0.61 U/microL Taq poly (Promega, Madison, WI)Cycler: N/A
Note: Used with HCV 368 to amplify a 213bp product.

Total 1 Oligos
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