A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Reverse(HCV 368) primer
Oligo ID:PB00490
Oligo type: Denegeracy: 1
Target: 389-373 Tm: 52 CLength: 17
Sequence: CCATGTGCCATGTACAG
(4 other experimental conditions)
Note: Used with BVD 100 to amplify a 290bp product.
Used with BVD 180 to amplify a 213bp product.

Experimental condition used for the oligonucleotide

Common Data ID: MP0257 Publication date: 10/00
PMID: 11131328 GI: 9629506 Taxonomy ID:31656
Virus Name: bovine viral diarrhea virus (strain SD-1), BVDV Product size (PCR): 290 bp
MgCl2: 4 mM dNTPs: 0.4 mM Type: RT-Nested PCR 1
Temp (PCR cycle):37C 45min>95C 5min>(94C 1min>55C 1min>72C 1min)*20>72C 10min
Buffer: 1x PCR buffer, 0.2% stock Triton X-100, 0.5mM dithiothreitol
Polymerase: 0.05 U/microL Taq poly (Promega, Madison, WI)Cycler: N/A
Note: Used with BVD 100 to amplify a 290bp product.
Used with BVD 180 to amplify a 213bp product.



Oligonucleotide information
Reverse(HCV 368) primer
Oligo ID:PB00490
Oligo type: Denegeracy: 1
Target: 389-373 Tm: 52 CLength: 17
Sequence: CCATGTGCCATGTACAG
(4 other experimental conditions)
Note: Used with BVD 100 to amplify a 290bp product.
Used with BVD 180 to amplify a 213bp product.

Experimental condition used for the oligonucleotide

Common Data ID: MP0258 Publication date: 10/00
PMID: 11131328 GI: 9629506 Taxonomy ID:31656
Virus Name: bovine viral diarrhea virus (strain SD-1), BVDV Product size (PCR): 213 bp
MgCl2: 4 mM dNTPs: 0.4 mM Type: RT- Nested PCR 2
Temp (PCR cycle):(94C 1min>55C 1min>72C 45 sec)*30>72C 10min
Buffer: Same as first PCR
Polymerase: 0.61 U/microL Taq poly (Promega, Madison, WI)Cycler: N/A
Note: Used with BVD 100 to amplify a 290bp product.
Used with BVD 180 to amplify a 213bp product.



Oligonucleotide information
Reverse(HCV 368) primer
Oligo ID:PB00490
Oligo type: Denegeracy: 1
Target: 389-373 Tm: 52 CLength: 17
Sequence: CCATGTGCCATGTACAG
(4 other experimental conditions)
Note: Used with BVD 100 to amplify a 290bp product.
Used with BVD 180 to amplify a 213bp product.

Experimental condition used for the oligonucleotide

Common Data ID: MP0257 Publication date: 10/00
PMID: 11131328 GI: 9629506 Taxonomy ID:31656
Virus Name: bovine viral diarrhea virus (strain SD-1), BVDV Product size (PCR): 290 bp
MgCl2: 4 mM dNTPs: 0.4 mM Type: RT-Nested PCR 1
Temp (PCR cycle):37C 45min>95C 5min>(94C 1min>55C 1min>72C 1min)*20>72C 10min
Buffer: 1x PCR buffer, 0.2% stock Triton X-100, 0.5mM dithiothreitol
Polymerase: 0.05 U/microL Taq poly (Promega, Madison, WI)Cycler: N/A
Note: Used with BVD 100 to amplify a 290bp product.
Used with BVD 180 to amplify a 213bp product.



Oligonucleotide information
Reverse(HCV 368) primer
Oligo ID:PB00490
Oligo type: Denegeracy: 1
Target: 389-373 Tm: 52 CLength: 17
Sequence: CCATGTGCCATGTACAG
(4 other experimental conditions)
Note: Used with BVD 100 to amplify a 290bp product.
Used with BVD 180 to amplify a 213bp product.

Experimental condition used for the oligonucleotide

Common Data ID: MP0258 Publication date: 10/00
PMID: 11131328 GI: 9629506 Taxonomy ID:31656
Virus Name: bovine viral diarrhea virus (strain SD-1), BVDV Product size (PCR): 213 bp
MgCl2: 4 mM dNTPs: 0.4 mM Type: RT- Nested PCR 2
Temp (PCR cycle):(94C 1min>55C 1min>72C 45 sec)*30>72C 10min
Buffer: Same as first PCR
Polymerase: 0.61 U/microL Taq poly (Promega, Madison, WI)Cycler: N/A
Note: Used with BVD 100 to amplify a 290bp product.
Used with BVD 180 to amplify a 213bp product.

Total 4 Oligos
Copyright © 2001-2005 VirOligo Database Project. All rights reserved Feedback is welcome. Contact webmaster Ulrich Melcher at ulrich.melcher@okstate.edu.