A Simple Solution for Oligonucleotide Design

Click here for a list of viruses in the database

Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

°C± °C
Oligo length:
bp± bp


Common Data ID:

Taxonomy ID:

GI Number:

Oligonucleotide information
Forward(BVD 100) primer
Oligo ID:PB00489
Oligo type: Denegeracy: 1
Target: 100-117 Tm: 58 CLength: 18
(4 other experimental conditions)
Note: Used with HCV 368 to amplify a 290bp product.

Experimental condition used for the oligonucleotide

Common Data ID: MP0257 Publication date: 10/00
PMID: 11131328 GI: 9629506 Taxonomy ID:31656
Virus Name: bovine viral diarrhea virus (strain SD-1), BVDV Product size (PCR): 290 bp
MgCl2: 4 mM dNTPs: 0.4 mM Type: RT-Nested PCR 1
Temp (PCR cycle):37C 45min>95C 5min>(94C 1min>55C 1min>72C 1min)*20>72C 10min
Buffer: 1x PCR buffer, 0.2% stock Triton X-100, 0.5mM dithiothreitol
Polymerase: 0.05 U/microL Taq poly (Promega, Madison, WI)Cycler: N/A
Note: Used with HCV 368 to amplify a 290bp product.

Total 1 Oligos
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