A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Forward(PF1) primer
Oligo ID:pb00413
Oligo type: Denegeracy: 4
Target: 108-127 Tm: Max Tm 74.1 C Min Tm 72 C at salt 1000.0mMLength: 20
Sequence: ATGCCC(A/T)(C/T)AGTAGGACTAGC
(4 other experimental conditions)
Note:

Experimental condition used for the oligonucleotide

Common Data ID: BM0039 Publication date: 12/96
PMID: 9088554 GI: 9626649 Taxonomy ID:11099
Virus Name: pestivirus type 1 Product size (PCR):
bp
MgCl2:
mM
dNTPs: 0.2 mM Type: RT-Nested PCR
Temp (PCR cycle):(94C 1min>56C 2min>72C 2min)*30
Buffer: 1x buffer (Perkin-Elmer Cetus)
Polymerase: 0.025U/microL Taq poly (Perkin-Elmer Cetus)Cycler:
Note:

Total 1 Oligos
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