A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Forward(coro3) primer
Oligo ID:MP10930
Oligo type: PCR Denegeracy: 1
Target: 325-341 Tm: 52 CLength: 17
Sequence: TACACACCTCAGCGTTG
(2 other experimental conditions)
Note:

Experimental condition used for the oligonucleotide

Common Data ID: MP1093 Publication date: 06/03
PMID: 12765993 GI: 30314341 Taxonomy ID:227859
Virus Name: SARS coronavirus, SARS Product size (PCR): 182 bp
MgCl2: 3.5 mM dNTPs: N/A mM Type: RT-PCR
Temp (PCR cycle):95C 10min>(95C 10sec>57C 5sec>72C 9sec)*50
Buffer: N/A
Polymerase: N/ACycler: LightCycler (Roche)
Note:



Oligonucleotide information
Forward(coro3) primer
Oligo ID:MP10930
Oligo type: PCR Denegeracy: 1
Target: 325-341 Tm: 52 CLength: 17
Sequence: TACACACCTCAGCGTTG
(2 other experimental conditions)
Note:

Experimental condition used for the oligonucleotide

Common Data ID: SG0128 Publication date: 10/03
PMID: 14532176 GI: 37960831 Taxonomy ID:227859
Virus Name: SARS coronavirus Product size (PCR): 182 bp
MgCl2: 2.5 mM dNTPs: 2.0 mM Type: PCR
Temp (PCR cycle):94C 600sec>(94C 30sec>50C 40sec>72C 15sec)*40>72C 600sec
Buffer: 1x reaction buffer
Polymerase: 2U AmpliTaq goldCycler:
Note:



Oligonucleotide information
Forward(coro3) primer
Oligo ID:MP10930
Oligo type: PCR Denegeracy: 1
Target: 325-341 Tm: 52 CLength: 17
Sequence: TACACACCTCAGCGTTG
(2 other experimental conditions)
Note:

Experimental condition used for the oligonucleotide

Common Data ID: MP1093 Publication date: 06/03
PMID: 12765993 GI: 30314341 Taxonomy ID:227859
Virus Name: SARS coronavirus, SARS Product size (PCR): 182 bp
MgCl2: 3.5 mM dNTPs: N/A mM Type: RT-PCR
Temp (PCR cycle):95C 10min>(95C 10sec>57C 5sec>72C 9sec)*50
Buffer: N/A
Polymerase: N/ACycler: LightCycler (Roche)
Note:



Oligonucleotide information
Forward(coro3) primer
Oligo ID:MP10930
Oligo type: PCR Denegeracy: 1
Target: 325-341 Tm: 52 CLength: 17
Sequence: TACACACCTCAGCGTTG
(2 other experimental conditions)
Note:

Experimental condition used for the oligonucleotide

Common Data ID: SG0128 Publication date: 10/03
PMID: 14532176 GI: 37960831 Taxonomy ID:227859
Virus Name: SARS coronavirus Product size (PCR): 182 bp
MgCl2: 2.5 mM dNTPs: 2.0 mM Type: PCR
Temp (PCR cycle):94C 600sec>(94C 30sec>50C 40sec>72C 15sec)*40>72C 600sec
Buffer: 1x reaction buffer
Polymerase: 2U AmpliTaq goldCycler:
Note:

Total 4 Oligos
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