A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Reverse(BD2) primer
Oligo ID:MP02651
Oligo type: PCR Denegeracy: 8
Target: 1104-1085 Tm: Max Tm 74.1 C Min Tm 67.9 C at salt 1000.0mMLength: 20
Sequence: TTGTT(A/G)TGGTACA(A/G)(A/G)CCGTC
(1 other experimental conditions)
Note:

Experimental condition used for the oligonucleotide

Common Data ID: MP0265 Publication date: 04/97
PMID: 9129644 GI: 7960753 Taxonomy ID:11100
Virus Name: bovine viral diarrhea virus (isolate NADL), BVDV Product size (PCR): 738 bp
MgCl2: 2.5 mM dNTPs: 0.2 mM Type: RT-PCR
Temp (PCR cycle):(94C 1min>52C 1min>72C 1min)*35>72C 7min
Buffer: 1x PCR buffer (10mM Tris-HCl, pH 9.0, 50mM KCl, 0.1mg/ml BSA)
Polymerase: 0.02U/microL Taq poly (Perkin Elmer-Cetus)Cycler: Perkin Elmer Cetus Thermocycler
Note:

Total 1 Oligos
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