A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Forward(p80.1) primer
Oligo ID:MP00800
Oligo type: PCR Denegeracy: 32
Target: N/A-N/A Tm: Max Tm 85.1 C Min Tm 79 C at salt 1000.0mMLength: 27
Sequence: TCGAGCTCGGATGAA(A/G)GG(A/C/G/T)GA(A/G)GA(T/C)CT
(0 other experimental conditions)
Note:

Experimental condition used for the oligonucleotide

Common Data ID: MP0080 Publication date: 08/91
PMID: 1656820 GI: N/A Taxonomy ID:11100
Virus Name: bovine viral diarrhea virus (isolate NADL), BVDV Product size (PCR): 562 bp
MgCl2: 2 mM dNTPs: 0.04 mM Type: RT-PCR
Temp (PCR cycle):94C 1min>37C 30sec>72C 10min>(94C 1min>37C 30sec>72C 30sec)*5>(94C 1min>55C 30sec>72C 30sec)*30
Buffer: 1xPCR buffer (50mM KCl, 10mM Tris, 0.01% gelatin, pH8.8)
Polymerase: 0.025U/microL Taq 1 poly (Amplitaq, Cetus Corp, Emeryville, Calif.)Cycler: Thermocycler (Perkin-Elmer/Cerus Corp. Norwalk, Conn.)
Note:

Total 1 Oligos
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