A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Reverse(Beta) primer
Oligo ID:MP00141
Oligo type: PCR Denegeracy: 1
Target:
-
Tm: 77.2 C at salt 1000.0mMLength: 25
Sequence: CGAATTCCATTGGCTTCATCCCACA
(0 other experimental conditions)
Note: This sequence coould not be found in the GI given but amplification of it and alpha led to a 2.571kb fragment.

Experimental condition used for the oligonucleotide

Common Data ID: MP0014 Publication date: 09/00
PMID: 11014610 GI: 261087 Taxonomy ID:10385
Virus Name: Bovine herpesvirus 4, BHV-4 Product size (PCR):
bp
MgCl2: 3 mM dNTPs: 0.25 mM Type: PCR
Temp (PCR cycle):94C 5min>(94C 1min>55C 1min>72C 2min)*30>72C 5min
Buffer: 10mM Tris HCl pH 8.3, 50mM KCl
Polymerase: 0.02 U/microL Taq Poly (Boehringer-Diagnostics, Milan, Italy)Cycler:
Note: This sequence coould not be found in the GI given but amplification of it and alpha led to a 2.571kb fragment.

Total 1 Oligos
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