A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Forward(MP00071) primer
Oligo ID:MP00070
Oligo type: PCR Denegeracy: 1
Target: 3234-3259 Tm: 74.2 C at salt 1000.0mMLength: 26
Sequence: ATTTAAGTGCACACCTCTTATTTGCG
(0 other experimental conditions)
Note: This sequence is not identicle to the one in the GI, the similarities are, the first 15 nucleotides are the same in order after that 3 are the same and 8 are different however if you also segments that are identicle but are 1 nucleotide ahead of the primer, ex. attt and ttt

Experimental condition used for the oligonucleotide

Common Data ID: MP0007 Publication date: 12/96
PMID: 9233501 GI: 330756 Taxonomy ID:10320
Virus Name: Bovine herpesvirus 1, BHV-1 Product size (PCR):
bp
MgCl2: 1.5 mM dNTPs: 0.2 mM Type: PCR
Temp (PCR cycle):(95C 1min>60C 1min>72C 2min)*35
Buffer: 10mM Tris-HCL pH 8.8, 50mM KCL, 0.1% Triton X-100
Polymerase: 0.02U/microL Taq DNA polyCycler:
Note: This sequence is not identicle to the one in the GI, the similarities are, the first 15 nucleotides are the same in order after that 3 are the same and 8 are different however if you also segments that are identicle but are 1 nucleotide ahead of the primer, ex. attt and ttt

Total 1 Oligos
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