A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Reverse(B4) primer
Oligo ID:MO00051
Oligo type: PCR Denegeracy: 1
Target: 360-343 Tm: 56 CLength: 18
Sequence: GTAGCAATACAGTGGGCC
(0 other experimental conditions)
Note: This primer is for BVDV type one. The target area was specified in the article and only for 17 base pairs

Experimental condition used for the oligonucleotide

Common Data ID: MO0005 Publication date: 01/99
PMID: 10028170 GI:
Taxonomy ID:144353
Virus Name: bovine viral diarrhea virus, BVDV-1, BVDV-2 Product size (PCR): 221 bp
MgCl2: 3 mM dNTPs: 0.5 mM Type: RT-PCR
Temp (PCR cycle):95C 5min>(94C 1min>52C 1min>72C 1min)*35
Buffer: 20mM Tris-HCl (pH 8.4); 50mM KCl
Polymerase: 0.05U/microL Taq poly (GIBCO BRL)Cycler:
Note: This primer is for BVDV type one. The target area was specified in the article and only for 17 base pairs

Total 1 Oligos
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