A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Reverse(GC1) primer
Oligo ID:KS00130
Oligo type: PCR Denegeracy: 8
Target: 18250-18228 Tm: Max Tm 89.3 C Min Tm 83.9 C at salt 1000.0mMLength: 23
Sequence: GCCGAGAG(A/C)CCGCC(A/G)G(A/C)CCGAGA
(1 other experimental conditions)
Note:

Experimental condition used for the oligonucleotide

Common Data ID: KS0013 Publication date: 12/99
PMID: 10598083 GI: 9629818 Taxonomy ID:79889
Virus Name: Bovine herpesvirus type 1.1, BHV-1; Bovine herpesvirus 5, BHV-5 Product size (PCR):
bp
MgCl2: 1.5 mM dNTPs: 0.2 mM Type: Nested PCR 2
Temp (PCR cycle):94C 9min>(95C 1min>67C 1min>72C 1min)*35
Buffer: 50mM KCl, 10mM Tris-HCl (pH 9.0), 5.0% DMSO
Polymerase: 0.02U/microL AmpliTaq Gold DNA poly (Perkin-Elmer)Cycler:
Note:

Total 1 Oligos
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