A Simple Solution for Oligonucleotide Design

Click here for a list of viruses in the database

Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

°C± °C
Oligo length:
bp± bp


Common Data ID:

Taxonomy ID:

GI Number:

Oligonucleotide information
Reverse(P2) primer
Oligo ID:HI00051
Oligo type: PCR Denegeracy: 1
Target: 2250-2222 Tm: 77.8 C at salt 1000.0mMLength: 29
(3 other experimental conditions)
Note: Mismatches at positions 2227, 2242, and 2246.

Experimental condition used for the oligonucleotide

Common Data ID: HI0005 Publication date: 10/95
PMID: 8578861 GI: 323205 Taxonomy ID:11100
Virus Name: bovine viral diarrhea virus (isolate NADL); pestivirus type 2 strain BVDV890; border disease virus strain BD31 Product size (PCR): 826 bp
MgCl2: 1.5 mM dNTPs: 1.0 mM Type: RT-Nested PCR 1
Temp (PCR cycle):50C 30min>94C 2min>(94C 1min>55C 1min>72C 1min)*30>72C 10min
Buffer: 50mM KCl, 10mM Tris-HCl (pH 8.3), 0.25mM dithiothreitol
Polymerase: 0.025U/microL Amplitaq (Perkin-Elmer Cetus)Cycler:
Note: Mismatches at positions 2227, 2242, and 2246.

Total 1 Oligos
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