A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Reverse(DFA) primer
Oligo ID:HI00042
Oligo type: PCR Denegeracy: 512
Target: 2149-2168 Tm: Max Tm 78.2 C Min Tm 63.8 C at salt 1000.0mMLength: 20
Sequence: GA(C/T)TT(C/T)GC(A/C/G/T)AG(C/T)(C/T)T(A/C/G/T)TA(C/T)CC
(2 other experimental conditions)
Note: Specific to [GI:59839].

Experimental condition used for the oligonucleotide

Common Data ID: HI0004 Publication date: 05/98
PMID: 9557713 GI: 59839 Taxonomy ID:10304
Virus Name: Bovine Lymphotropic Herpesvirus, BLHV Product size (PCR):
bp
MgCl2: 2 mM dNTPs: 0.2 mM Type: Nested PCR
Temp (PCR cycle):94C 3min>60C 2min>72C 1min>(94C 30sec>46C 1min>72C 1min)*44
Buffer: 20mM Tris-HCl, pH8.4, 50mM KCl, 2.5% dimethyl sulfoxide
Polymerase: 0.05U/microL Tag ploy(Gibco)Cycler: Omnigene thermal cycler(Hybaid Limited, Middlesex, UK)
Note: Specific to [GI:59839].



Oligonucleotide information
Reverse(DFA) primer
Oligo ID:HI00042
Oligo type: PCR Denegeracy: 512
Target: 2149-2168 Tm: Max Tm 78.2 C Min Tm 63.8 C at salt 1000.0mMLength: 20
Sequence: GA(C/T)TT(C/T)GC(A/C/G/T)AG(C/T)(C/T)T(A/C/G/T)TA(C/T)CC
(2 other experimental conditions)
Note: Specific to [GI:59839].

Experimental condition used for the oligonucleotide

Common Data ID: HI0003 Publication date: 05/98
PMID: 9557713 GI: 59839 Taxonomy ID:69955
Virus Name: bovine lymphotropic herpesvirus, BLHV; Ovine herpesvirus 2, OHV2; Bovine herpesvirus 4, BHV4 Product size (PCR):
bp
MgCl2: 2.0 mM dNTPs: 0.2 mM Type: Nested PCR
Temp (PCR cycle):94C 3min>60C 2min>72C 1min>(94C 30sec>60C 60sec>72C 60sec)*35
Buffer: 20mM Tris HCl (pH8.4), 50mM KCl, 2.5% dimethyl sulfoxide
Polymerase: 0.05U/microL Taq poly (Gibco)Cycler: Omnigene thermal cycler (Hybaid Limited, Middlesex, UK)
Note: Specific to [GI:59839].



Oligonucleotide information
Reverse(DFA) primer
Oligo ID:HI00042
Oligo type: Denegeracy: 512
Target: 2149-2168 Tm: Max Tm 78.2 C Min Tm 63.8 C at salt 1000.0mMLength: 20
Sequence: GA(C/T)TT(C/T)GC(A/C/G/T)AG(C/T)(C/T)T(A/C/G/T)TA(C/T)CC
(2 other experimental conditions)
Note: Specific to [GI:59839].

Experimental condition used for the oligonucleotide

Common Data ID: HI0004 Publication date: 05/98
PMID: 9557713 GI: 59839 Taxonomy ID:10304
Virus Name: Bovine Lymphotropic Herpesvirus, BLHV Product size (PCR):
bp
MgCl2: 2 mM dNTPs: 0.2 mM Type: Nested PCR
Temp (PCR cycle):94C 3min>60C 2min>72C 1min>(94C 30sec>46C 1min>72C 1min)*44
Buffer: 20mM Tris-HCl, pH8.4, 50mM KCl, 2.5% dimethyl sulfoxide
Polymerase: 0.05U/microL Tag ploy(Gibco)Cycler: Omnigene thermal cycler(Hybaid Limited, Middlesex, UK)
Note: Specific to [GI:59839].



Oligonucleotide information
Reverse(DFA) primer
Oligo ID:HI00042
Oligo type: Denegeracy: 512
Target: 2149-2168 Tm: Max Tm 78.2 C Min Tm 63.8 C at salt 1000.0mMLength: 20
Sequence: GA(C/T)TT(C/T)GC(A/C/G/T)AG(C/T)(C/T)T(A/C/G/T)TA(C/T)CC
(2 other experimental conditions)
Note: Specific to [GI:59839].

Experimental condition used for the oligonucleotide

Common Data ID: HI0003 Publication date: 05/98
PMID: 9557713 GI: 59839 Taxonomy ID:69955
Virus Name: bovine lymphotropic herpesvirus, BLHV; Ovine herpesvirus 2, OHV2; Bovine herpesvirus 4, BHV4 Product size (PCR):
bp
MgCl2: 2.0 mM dNTPs: 0.2 mM Type: Nested PCR
Temp (PCR cycle):94C 3min>60C 2min>72C 1min>(94C 30sec>60C 60sec>72C 60sec)*35
Buffer: 20mM Tris HCl (pH8.4), 50mM KCl, 2.5% dimethyl sulfoxide
Polymerase: 0.05U/microL Taq poly (Gibco)Cycler: Omnigene thermal cycler (Hybaid Limited, Middlesex, UK)
Note: Specific to [GI:59839].

Total 4 Oligos
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