A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Forward(alpha) primer
Oligo ID:HI00010
Oligo type: PCR Denegeracy: 1
Target: -1-24 Tm: 72.3 C at salt 1000.0mMLength: 25
Sequence: CGAATTATAGTCTAAAGTCATCCTC
(1 other experimental conditions)
Note: First bp does not complement GI.
Mismatch at position 6.

Experimental condition used for the oligonucleotide

Common Data ID: HI0001 Publication date: 12/00
PMID: 11101621 GI: 261087 Taxonomy ID:10385
Virus Name: Bovine herpesvirus 4, BoHV-4 Product size (PCR): 2538 bp
MgCl2: 3.0 mM dNTPs: 0.2 mM Type: PCR
Temp (PCR cycle):94C 5min>(94C 1min>55C 1min>72C 2min)*30>72C 5min
Buffer: 10mM Tris-HCl (pH 8.3), 50mM KCl
Polymerase: 0.02U/microL Taq poly (Boehringer Diagnostics, Milan, Italy)Cycler:
Note: First bp does not complement GI.
Mismatch at position 6.

Total 1 Oligos
Copyright © 2001-2005 VirOligo Database Project. All rights reserved Feedback is welcome. Contact webmaster Ulrich Melcher at ulrich.melcher@okstate.edu.