A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Reverse(F4) primer
Oligo ID:BM00011
Oligo type: PCR Denegeracy: 1
Target: 673-656 Tm: 50 CLength: 18
Sequence: GACACTATCTTAAGGTTC
(0 other experimental conditions)
Note: Used in combination with G4 or G1 in PCR.

Experimental condition used for the oligonucleotide

Common Data ID: BM0001 Publication date: 10/98
PMID: 9850996 GI: 1850942 Taxonomy ID:82823
Virus Name: bovine respiratory syncytial virus strain lelystad, BRSV strain Lelystad Product size (PCR):
bp
MgCl2: 2.4 mM dNTPs: 0.29 mM Type: RT-PCR
Temp (PCR cycle):98C 10min>(94C 1min>55C 1min>72C 1min)*35
Buffer: 12.5mM Tris-HCl, 51.5mM KCl, 10microg/mL gelatine
Polymerase: 0.025U/microL Taq Poly (Boehringer)Cycler: DNA thermal cycler (Omnigene)
Note: Used in combination with G4 or G1 in PCR.

Total 1 Oligos
Copyright © 2001-2005 VirOligo Database Project. All rights reserved Feedback is welcome. Contact webmaster Ulrich Melcher at ulrich.melcher@okstate.edu.