A Simple Solution for Oligonucleotide Design


Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:



Oligonucleotide information
Reverse(F1) primer
Oligo ID:AM00191
Oligo type: PCR Denegeracy: 1
Target: 28-12 Tm: 86.8 C at salt 1000.0mMLength: 26
Sequence: CACGGATCCGGCTGTTGCCGCCATCC
(0 other experimental conditions)
Note: Primer from the 5' end of the F gene.
BLAST resulted in a mismatch of the first 9 nucleotides from the 5' end.
GI # 210828.

Experimental condition used for the oligonucleotide

Common Data ID: AM0019 Publication date: 08/98
PMID: 9880007 GI: 2117036 Taxonomy ID:11246
Virus Name: Bovine respiratory syncytial virus, BRSV Product size (PCR): 417 bp
MgCl2: 2.18 mM dNTPs: 0.2 mM Type: RT-PCR
Temp (PCR cycle):(94C 1min>60C 1min>72C 90sec)*35>72C 5min30sec
Buffer: 10mM Tris-HCl[pH 9.0], 50mM KCl
Polymerase: 0.02U/microL Taq poly(Perkin Elmer)Cycler: Perkin Elmer Thermocyclers
Note: Primer from the 5' end of the F gene.
BLAST resulted in a mismatch of the first 9 nucleotides from the 5' end.
GI # 210828.

Total 1 Oligos
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