A Simple Solution for Oligonucleotide Design

Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: UM0018Publication date: 8/2003
PMID: 12690091GI: 30271926 Taxonomy ID: 227859
Virus Name: SARS CoronavirusProduct size (PCR): 108 bp
MgCl2: 0.42 mMdNTPs: mMType: Nested PCR
Temp (PCR cycle):95C 3min>(95C 10sec>60C 10sec[-1C/cyc]>72C 20sec)*10>(95C 10sec>56C 10sec>72C 20sec)*20
Buffer:
Polymerase: Cycler:
Note:
Forward(SAR1S) primer
Oligo type: PCRDegeneracy: 1
Target: 15271-15291Tm: 74.4 C at salt 1000.0mMLength:
Sequence: CCTCTCTTGTTCTTGCTCGCA
(3 other experimental conditions)
Note: Target range from sequence.
Reverse(SAR1As) primer
Oligo type: PCRDegeneracy: 1
Target: 15391-15371Tm: 74.4 C at salt 1000.0mMLength:
Sequence: TATAGTGAGCCGCCACACATG
(3 other experimental conditions)
Note: Target range from sequence.
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