A Simple Solution for Oligonucleotide Design

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Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



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Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: UM0016Publication date: 8/2003
PMID: 12690091GI: 12690091 Taxonomy ID: 227859
Virus Name: SARS CoronavirusProduct size (PCR): 440 bp
MgCl2: N/A mMdNTPs: mMType: RT-PCR
Temp (PCR cycle):45C 30min>95C 3min(95C 10sec>60C 10sec[-1C/cyc]>72C 30sec)*10>(95C 10sec>56C 10sec>72C 30sec)*40
Buffer: Superscript II reverse transcriptase (RT)/platinum Taq pol one-step RT-PCR (InVitrogen)
Polymerase: Platinum Taq polyCycler: N/A
Note: Reactions were conducted in 20microL total volume containing: 10microL 2X reaction buffer, 2.45mM magnesium sulfate, 500nM each primer, 0.4microL reverse-transcriptase/Taq DNA polymerase mixture and 2microL RNA.

For a nested PCR using this reaction for first round amplification, [see UM0018]. For other PCRs from this article [see UM0015], [see UM0017], [see UM0019], [see UM0020].
Forward(IN-6) primer
Oligo type: PCRDegeneracy: 1
Target: 14325-14348Tm: 77 C at salt 1000.0mMLength:
Sequence: GGTTGGGGACTATCCTAAGTGTGA
(0 other experimental conditions)
Note: Target range from gi 12082738
Forward(IN-7) primer
Oligo type: PCRDegeneracy: 1
Target: 14583-14560Tm: 70.2 C at salt 1000.0mMLength:
Sequence: CCATCATCAGATAGAATCATCATA
(1 other experimental conditions)
Note: Target range from gi 133979001
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