A Simple Solution for Oligonucleotide Design

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2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: SW0086Publication date: 11/00
PMID: 11044116GI: 450251 Taxonomy ID: 11246
Virus Name: Bovine respiratory syncytial virus, BRSVProduct size (PCR):
bp
MgCl2: 1.0 mMdNTPs: mMType: RT-nested PCR 2
RT-Nested PCR 1 RT-Nested PCR 1 RT-Nested PCR 2 RT-nested PCR 2
Temp (PCR cycle):94C 12min>(94C 45sec>49C 60sec>72C 60sec)*35>72C 10min
Buffer: 10mM Tris-HCl (pH 8.3), 50mM KCl
Polymerase: 0.025U/microL AmpliTaq Gold poly (Perkin-Elmer)Cycler: GeneAmp PCR system 480 (Perkin-Elmer)
Note: For RT and primary PCR, [see SW0085].

Total PCR volume is 100microL containing 10microL primary PCR product (1:10) and 0.5microM each primer.
Article cites 200mM each dNTP being used in PCR.

For other PCRs in this article, [see SW0087], [see SW0088], and [see SW0089].
Forward(N2.3) primer
Oligo type: PCRDegeneracy: 1
Target: 127-146Tm: 67.9 C at salt 1000.0mMLength:
Sequence: CATCTCAATAAGTTGTGTGG
(1 other experimental conditions)
Note: Specific to N gene.
Reverse(N2.4) primer
Oligo type: PCRDegeneracy: 1
Target: 857-838Tm: 67.9 C at salt 1000.0mMLength:
Sequence: TCTACAACCTGTTCCATTTC
(1 other experimental conditions)
Note: Specific to N gene.
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