A Simple Solution for Oligonucleotide Design

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Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

°C± °C
Oligo length:
bp± bp


Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: SW0040Publication date: 07/97
PMID: 9403511GI: 9626649 Taxonomy ID: 11099
Virus Name: pestivirus type 1, BVDVProduct size (PCR):
MgCl2: 1.5 mMdNTPs: mMType: RT-PCR
Temp (PCR cycle):(94C 30sec>55C 100sec>72C 100sec)*30
Buffer: 16mM Tris-HCl, 98mM KCl, 500ng/microL BSA, 0.1% sodium cholate, 0.1% Triton X-100, 0.06mM DTT, 6microM disodium ethylenediaminetetraacetate, 3% glycerol
Polymerase: 0.025U/microL Tth DNA poly (Toyobo Co.; Tokyo, Japan)Cycler: TSR-300 thermal cycler (Iwaki Glass Co.; Tokyo, Japan)
Note: Total RT volume is 40microL containing 8microL RNA, 50mM Tris-HCl (pH 8.3), 75mM KCl, 3.0mM MgCl2, 40mM DTT, 0.625U/microL MMLV RTase (Gibco-BRL), 0.16mM dNTPs (Gibco-BRL), 0.55U/microL ribonuclease inhibitor (Takara Shuzo Co.), and 0.25microM primer R.
RT temperature: 37C 90min.

Total PCR volume is 50microL containing 6microL cDNA and 0.2microM each primer.
Forward(F) primer
Oligo type: PCRDegeneracy: 4
Target: 108-127Tm: Max Tm 74.1 C Min Tm 72 C at salt 1000.0mMLength:
(4 other experimental conditions)
Reverse(R) primer
Oligo type: PCRDegeneracy: 1
Target: 392-373Tm: 72 C at salt 1000.0mMLength:
(5 other experimental conditions)
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