A Simple Solution for Oligonucleotide Design

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Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: SK0062Publication date: 2000
PMID: 10971126GI: 9629818 Taxonomy ID: 10320
Virus Name: Bovine herpesvirus 1, BHV-1Product size (PCR): 179 bp
MgCl2: 1.5 mMdNTPs: mMType: PCR
Temp (PCR cycle):97C 3min>61C 3min>72C 3min>(97C 1min>61C 1min>72C 1min)*20>(97C 30sec>61C 30sec>72C 30sec)*40
Buffer: 1x PCR buffer (Gibco BRL)
Polymerase: 0.025U/microL Taq DNA poly (Gibco BRL)Cycler: GeneAmp PCR System 2400 (Perkin-Elmer)
Note: Total PCR volume is 50microL containing 3microL DNA and 0.5microM each primer.

Article performs this PCR with 0.03-8.0fg/microL competitor plasmid pCBTK to quantify viral DNA. Primers amplify a 273bp-product from this plasmid.
Forward(BTKF2) primer
Oligo type: Degeneracy: 1
Target: 62810-62828Tm: 73.7 C at salt 1000.0mMLength:
Sequence: TGGGAGACGAACACCTGCA
(0 other experimental conditions)
Note: Mismatch at position 62825.
Reverse(BTKR1) primer
Oligo type: Degeneracy: 1
Target: 62987-62968Tm: 72 C at salt 1000.0mMLength:
Sequence: GTCTTATCGTGGAGCTGAAG
(0 other experimental conditions)
Note:
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