A Simple Solution for Oligonucleotide Design

Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: SG0128Publication date: 10/03
PMID: 14532176GI: 37960831 Taxonomy ID: 227859
Virus Name: SARS coronavirusProduct size (PCR): 182 bp
MgCl2: 2.5 mMdNTPs: mMType: PCR
Temp (PCR cycle):94C 600sec>(94C 30sec>50C 40sec>72C 15sec)*40>72C 600sec
Buffer: 1x reaction buffer
Polymerase: 2U AmpliTaq goldCycler:
Note: The reaction mix also contained 2microL of RT product.
Forward(coro3) primer
Oligo type: PCRDegeneracy: 1
Target: 325-341Tm: 52 CLength:
Sequence: TACACACCTCAGCGTTG
(2 other experimental conditions)
Note:
Reverse(coro4) primer
Oligo type: PCRDegeneracy: 1
Target: 506-491Tm: 48 CLength:
Sequence: CACGAACGTGACGAAT
(3 other experimental conditions)
Note:
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