A Simple Solution for Oligonucleotide Design

Click here for a list of viruses in the database

Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

°C± °C
Oligo length:
bp± bp


Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: SG0112Publication date: 08/03
PMID: 12904397GI: 623595 Taxonomy ID: 12870
Virus Name: Variola major virusProduct size (PCR): bp
MgCl2: 7 mMdNTPs: mMType: Real-time PCR
Temp (PCR cycle):95C 120sec>(95C 1sec>60C 20sec)*45
Buffer: 50mM Tris (pH 8.3), 25microG bovine serum albumin per mL
Polymerase: 0.8U Platinum TaqDNA (Invitrogen)Cycler: LightCycler
Note: The reaction mix also included 5microL of template DNA and 75nM TaqMan probe. A fluorescence reading was taken after each 60C step, and for each coded sample tested there was at least 1 positive control of 5fg of virus and one no template control.
Forward(OPXJ7R3U) primer
Oligo type: Forward PrimerDegeneracy: 1
Target: 151629-151649Tm: 70.5C at salt 1000.0 mMLength:
(1 other experimental conditions)
Reverse(OPXJ7R3L) primer
Oligo type: Reverse PrimerDegeneracy: 1
Target: 151767-151748Tm: 67.9C at salt 1000.0 mMLength:
(1 other experimental conditions)
Forward(VARJ7R3P) primer
Oligo type: PCR ProbeDegeneracy: 1
Target: 151652-151677Tm: 75.8C at salt 1000.0 mMLength:
(1 other experimental conditions)
Note: The probe contains a 6-carboxyfluorescein at the 5' end and a 6-carboxytetramethylrhodamine and a phosphate at the 3' end.
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