A Simple Solution for Oligonucleotide Design

Click here for a list of viruses in the database


Two search options:
1. Search by virus name and/or primer data
2. Search by PMID, VirOligoID, or taxonomy ID;

Virus name:

Oligo Sequence:

Tm:
°C± °C
Oligo length:
bp± bp



PMID:

Common Data ID:

Taxonomy ID:

GI Number:

CommonData ID: SG0078Publication date: 09/03
PMID: 14519240GI: 34420896 Taxonomy ID: 162145
Virus Name: Human metapneumovirusProduct size (PCR): bp
MgCl2: mMdNTPs: mMType: Nested RT-PCR
Temp (PCR cycle):50C 1800sec>94C 180sec>(94C 30sec>52C 30sec>72C 45sec)*40>72C 420sec
Buffer:
Polymerase: Cycler:
Note: The reaction was carried out using a single tube Superscript One-Step RT-PCR and Platinum Taq poly (Invitrogen). For the second Nested round of amplification Hot-Start DNA poly (HotStarTaq, QIAGEN) was used. So the thermal cycle was changed to 95C 900sec for the denaturation step.
Forward(SG007801) primer
Oligo type: Forward PrimerDegeneracy: 1
Target: 4231-4249Tm: 71.5C at salt 1000.0 mMLength:
Sequence: AGCTGTTCCATTGGCAGCA
(0 other experimental conditions)
Note: Outer Primer
Reverse(SG007802) primer
Oligo type: Reverse PrimerDegeneracy: 4
Target: 4371-4351Tm: 70.5C at salt 1000.0 mMLength:
Sequence: ATGCTGTTC(A/G)CC(C/T)TCAACTTT
(0 other experimental conditions)
Note: Outer Primer
Forward(SG007803) primer
Oligo type: Forward PrimerDegeneracy: 1
Target: 4253-4271Tm: 69.3C at salt 1000.0 mMLength:
Sequence: GAGTAGGGATCATCAAGCA
(0 other experimental conditions)
Note: Nested primer
Reverse(SG007804) primer
Oligo type: Reverse PrimerDegeneracy: 2
Target: 4350-4330Tm: 68.5C at salt 1000.0 mMLength:
Sequence: GCTTAGCTG(A/G)TATACAGTGTT
(0 other experimental conditions)
Note: Nested primer
Copyright © 2001-2005 VirOligo Database Project. All rights reserved Feedback is welcome. Contact webmaster Ulrich Melcher at ulrich.melcher@okstate.edu.